|
| Status |
Public on May 01, 2014 |
| Title |
PBMC stimulated with MTB.24h, rep16 |
| Sample type |
RNA |
| |
|
| Source name |
PBMCs, Mycobacterium, 24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: peripheral blood mononuclear cells (PBMCs)
disease status: healthy
treatment: Mycobacterium tuberculosis (MTB)
treatment duration: 24h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol using the Illumina iScan.
|
| Description |
Replicate 16
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R.
|
| |
|
| Submission date |
Nov 29, 2012 |
| Last update date |
May 01, 2014 |
| Contact name |
Aylwin Ng |
| Organization name |
CCIB, MGH
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE42606 |
Genome-wide transcriptional profiling of human PBMCs stimulated with Candida albicans and non-fungal inflammatory stimuli |
|
