|
| Status |
Public on Sep 01, 2006 |
| Title |
HUVEC/NmenMC58_pilD_1h_rep2_1107 |
| Sample type |
RNA |
| |
|
| Source name |
Human Umbilical Vein Endothelial Cells (HUVEC)
|
| Organism |
Homo sapiens |
| Characteristics |
Tissue: endothelial cells
|
| Biomaterial provider |
PromoCell Cat. no. C-12200-human umbilical vein endothelial cells; INSERM U570, Faculté de Médecine René Descartes, Paris, France, prof. Xavier Nassif - Neisseria meningitidis MC58
|
| Treatment protocol |
Monolayers of HUVECs in 9 cm Petri dishes infected with Neisseria meningitidis MC58 delta pilD were washed and collected into Trizol by cell-scraper
|
| Growth protocol |
Confluent monolayers of HUVECs in 9 cm Petri dishes were infected with Neisseria meningitidis MC58 delta pilD (MOI=10) and cultivated in RPMI medium, 5% CO2, humidified atmosphere, 37°C, 1 hour
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. Total RNA was purified on RNeasy minicolumns (Qiagen) and by NaOAc/ethanol precipitation.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized for 16 hr at 45C on Affymetrix Human Genome HG-U133A GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray scanner.
|
| Description |
Gene expression data from HUVEC infected with Neisseria meningitidis MC58 delta pilD, 1 hour infection
|
| Data processing |
The data were analyzed with RMA and MAS 5.0 algorithms using Biocondutor 1.7 and default analysis settings.
|
| |
|
| Submission date |
Apr 10, 2006 |
| Last update date |
Apr 10, 2006 |
| Contact name |
Marek Basler |
| E-mail(s) |
basler@biomed.cas.cz
|
| Phone |
+420 2 4106 2762
|
| URL |
http://l125lsx.mbu.cas.cz/Lab125/index.html
|
| Organization name |
Institute of Microbiology, Czech Academy of Sciences
|
| Department |
Cell and Molecular Microbiology Division
|
| Lab |
Laboratory of Molecular Biology of Bacterial Pathogens
|
| Street address |
Videnska 1083
|
| City |
Prague 4 |
| ZIP/Postal code |
CZ-142 20 |
| Country |
Czech Republic |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE4646 |
Signaling of Neisseria meningitidis MC58 mutants to primary human umbilical vein endothelial cells (HUVEC) |
|
