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Sample GSM10442 Query DataSets for GSM10442
Status Public on Jan 01, 2005
Title WT Ovule 2
Sample type RNA
 
Source name Genotype: Ws-0, Wild Type; Stage: Unfertilized Ovule; Biological Replica: 2 of 2
Organism Arabidopsis thaliana
Extracted molecule total RNA
 
Description Growth Condition: Plants were grown in a Conviron chamber under continuous light with fluorescent lamps at 20oC and 50% - 70% relative humidity. Experiments were performed as described (Le et. al., 2003, PNAS).


Microarray Methods: Total RNA was extracted using the Hot Borate Method (ref.). Fifteen micrograms of biotinylated complementary RNA (cRNA) was prepared as described (Le et. al., 2003, PNAS). cRNA was subsequently hybridized to Affymetrix ATH1 Arabidopsis GeneChips. The scanned array images were analyzed using Affymetrix Microarray Suite 5.0 (MAS 5.0) with a global scaling intensity set at 500.
Keywords = Arabidopsis
Keywords = seed development
Lot batch = 2003282
 
Submission date Sep 24, 2003
Last update date Aug 28, 2018
Contact name Bob Goldberg
E-mail(s) bobglab@mcdb.ucla.edu
Phone 310-825-3270
Organization name University of California, Los Angeles
Department Molecular, Cell and Developmental Biology
Street address 610 Charles E Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platform ID GPL198
Series (1)
GSE680 Transcript Profiling of Arabidopsis Plant Life Cycle
Relations
Reanalyzed by GSE119083

Data table header descriptions
ID_REF
VALUE This is the final calculated measurement for each probe set identifier that has been made comparable across all samples and rows
ABS_CALL A qualitative measurement indicating if the probe set is detected (Present ; P), not detected (Absent; A), or marginally detected (Marginal; M)
DETECTION P-VALUE A p-value indicating the significance of the Detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 546.8 P 0.00007
AFFX-BioB-M_at 986.2 P 0.000044
AFFX-BioB-3_at 859.9 P 0.000095
AFFX-BioC-5_at 1686.1 P 0.000044
AFFX-BioC-3_at 855.7 P 0.00006
AFFX-BioDn-5_at 1470.2 P 0.000044
AFFX-BioDn-3_at 6164.6 P 0.000044
AFFX-CreX-5_at 13572.9 P 0.000044
AFFX-CreX-3_at 16972.7 P 0.000044
AFFX-DapX-5_at 4.9 A 0.659339
AFFX-DapX-M_at 9.3 A 0.843268
AFFX-DapX-3_at 6.6 A 0.645547
AFFX-LysX-5_at 2.8 A 0.686277
AFFX-LysX-M_at 6 A 0.945787
AFFX-LysX-3_at 27.5 A 0.227636
AFFX-PheX-5_at 2.2 A 0.984817
AFFX-PheX-M_at 1.6 A 0.9273
AFFX-PheX-3_at 8.9 A 0.772364
AFFX-ThrX-5_at 6 A 0.916408
AFFX-ThrX-M_at 8.2 A 0.834139

Total number of rows: 22810

Table truncated, full table size 587 Kbytes.




Supplementary file Size Download File type/resource
GSM10442.CEL.gz 3.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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