|
| Status |
Public on Dec 03, 2013 |
| Title |
KhES1(p30)_undiff_hES |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated hES
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: undiff_hES
cell origin: blastocyst
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by Trizol reagent (Life Technologies) according to the manufacturer's instruction.
|
| Label |
Cy3
|
| Label protocol |
Agilent standard protocol
|
| |
|
| Hybridization protocol |
Agilent standard protocol
|
| Scan protocol |
Agilent standard protocol
|
| Data processing |
The data were analyzed using the Gene Spring GX 11.5.1 software program (Agilent Technologies).The data processing was performed as follows; (i) Threshold raw signals were set to 1.0, (ii) Log base 2 transformation was performed, (iii) 90th percentile normalization was chosen as the normalized algorithm.
|
| |
|
| Submission date |
Nov 21, 2012 |
| Last update date |
Dec 03, 2013 |
| Contact name |
Shinya Yamanaka |
| E-mail(s) |
yamanaka@cira.kyoto-u.ac.jp
|
| Phone |
81-75-366-7041
|
| Organization name |
Center for iPS Cell Research and Applicaton (CiRA), Kyoto University
|
| Street address |
53 Kawahara-machi, Shogoin, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8507 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10850 |
| Series (2) |
| GSE42446 |
Comparison of miRNA expression profilings between hESCs and hiPSCs |
| GSE49053 |
Differentiation defective phenotypes revealed by large scale analyses of human pluripotent stem cells |
|
