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Sample GSM102694

Query DataSets for GSM102694

Status

Public on Nov 15, 2006

Title

Tiling ChIP-CHIP Tup12-GFP

Sample type

genomic

Source name

Tup12-GFP

Organism

Schizosaccharomyces pombe

Characteristics

CHIP IP using Anti GFP antibody

Extracted molecule

genomic DNA

Label

Biotin

Description

For the GeneChip® S. pombe Tiling Array from Affymetrix the DNA was amplified to obtain 5 micrograms of DNA then fragmented around 100 base pairs using DNAse I, labelled with biotin and hybridized according to Affymetrix recommendations (Lengronne et al., 2004). Antibodies specific for GFP (Applied Biosciences 8372-2) for each IP.

Data processing

ChIP-chip Affymetrix signals per chip normalized to the 50th percentile

Submission date

Mar 31, 2006

Last update date

Jun 20, 2006

Contact name

Anthony Wright

E-mail(s)

yongtao.xue.franzen@ki.se

Organization name

Karolinska Institute

Lab

Anthony Wright

Street address

Blickagången 6

City

Huddinge

ZIP/Postal code

141 57

Country

Sweden

Platform ID

GPL2862

Series (2)

GSE4566	Role of transcriptional co-repressor proteins Ssn6, Tup11 and Tup12
GSE36429	Tiling ChIP-CHIP Ssn6-GFP, Tp11-GFP, Tup12-GFP; Hst4 vs WT Expression

Data table header descriptions
ID_REF
VALUE	ChIP-chip Affymetrix signals per chip normalized to the 50th percentile

Data table
ID_REF	VALUE
2-0_at	1.87
2-250_at	2.23
2-500_at	1.26
2-750_at	1.38
2-1000_at	1.02
2-1250_at	1.75
2-1500_at	1.36
2-1750_at	2.06
2-2000_at	1.23
2-2250_at	1.35
2-2500_at	0.71
2-2750_at	0.63
2-3000_at	1.07
2-3250_at	1.25
2-3500_at	1.42
2-3750_at	2.08
2-4000_at	2.43
2-4250_at	2.06
2-4500_at	1.76
2-4750_at	1.48

Total number of rows: 23061

Table truncated, full table size 394 Kbytes.

Supplementary data files not provided