|
| Status |
Public on Feb 28, 2014 |
| Title |
Control B |
| Sample type |
RNA |
| |
|
| Source name |
Control B
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: bone marrow
cell type: CD34+/CD71(high)/CD45- erythroid progenitors
disease status: healthy control
|
| Treatment protocol |
Primary CD34+/CD71(high)/CD45- mononuclear cells of the bone marrow of DBA patients (with RPS19 mutations) and controls were harvested and sorted.
|
| Growth protocol |
Primary patient samples that did not involve cell culture.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNA purification
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix label protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix HG-U133A arrays were hybridized with purified RNA samples.
|
| Scan protocol |
Signal amplication on Affymetrix Fluidics station 400
|
| Description |
Control B
|
| Data processing |
Microarray data were processed with RMA from the affy package in Bioconductor using custom probeset definitions for NCBI Entrez Genes derived from the method of Dai M et al (PMID: 16284200): HG-U133A Version 16 Custom CDF.
|
| |
|
| Submission date |
Oct 24, 2012 |
| Last update date |
Feb 28, 2014 |
| Contact name |
Vijay G Sankaran |
| Organization name |
Boston Children's Hospital
|
| Street address |
300 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL16212 |
| Series (1) |
| GSE41817 |
Gene expression analysis from erythroid progenitors of patients with Diamond-Blackfan anemia |
|
