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Sample GSM102104

Query DataSets for GSM102104

Status

Public on May 31, 2006

Title

CD4+ CD25+_2 (HG-U133B)

Sample type

RNA

Source name

CD4+CD25+

Organism

Homo sapiens

Characteristics

genetic modification:
tissue: Blood

Growth protocol

ex vivo

Extracted molecule

total RNA

Extraction protocol

Trizol

Label

biotin

Label protocol

Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

Hybridization protocol

Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Scan protocol

GeneChips were scanned using the Affymetrix GCS 3000.

Description

CD4+CD25+_2

Data processing

The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

Submission date

Mar 29, 2006

Last update date

May 31, 2006

Contact name

Robert Geffers

E-mail(s)

robert.geffers@helmholtz-hzi.de

Phone

+49 531-6181-3058

Organization name

HCI - Helmholtz Centre for Infection Research

Department

Dep. Molecular Bacteriology

Lab

Genome Analytics

Street address

Inhoffenstr. 7

City

Braunschweig

ZIP/Postal code

38124

Country

Germany

Platform ID

GPL97

Series (1)

GSE4571

Expression profiling of Treg cell versus their controls in human and mouse

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	690.1	P	0.000297
AFFX-BioB-M_at	1236.6	P	0.000169
AFFX-BioB-3_at	680.6	P	0.000225
AFFX-BioC-5_at	1741.3	P	0.000052
AFFX-BioC-3_at	1743.1	P	0.00007
AFFX-BioDn-5_at	2433.9	P	0.000857
AFFX-BioDn-3_at	9948.7	P	0.000044
AFFX-CreX-5_at	16687.6	P	0.000044
AFFX-CreX-3_at	29088.8	P	0.000044
AFFX-DapX-5_at	10.3	A	0.834139
AFFX-DapX-M_at	17.8	A	0.852061
AFFX-DapX-3_at	3.9	A	0.98333
AFFX-LysX-5_at	58.2	A	0.327079
AFFX-LysX-M_at	9.9	A	0.794268
AFFX-LysX-3_at	15.5	A	0.470241
AFFX-PheX-5_at	44.1	A	0.470241
AFFX-PheX-M_at	20.8	A	0.712257
AFFX-PheX-3_at	90.4	A	0.300606
AFFX-ThrX-5_at	3.3	A	0.984817
AFFX-ThrX-M_at	52.9	A	0.216524

Total number of rows: 22645

Table truncated, full table size 581 Kbytes.

Supplementary data files not provided