GEO Accession viewer

NCBI > GEO > Accession Display

Not logged in | Login

GEO help: Mouse over screen elements for information.

Sample GSM102103

Query DataSets for GSM102103

Status

Public on May 31, 2006

Title

CD4+ CD25+_2 (HG-U133A)

Sample type

RNA

Source name

CD4+CD25+

Organism

Homo sapiens

Characteristics

genetic modification:
tissue: Blood

Growth protocol

ex vivo

Extracted molecule

total RNA

Extraction protocol

Trizol

Label

biotin

Label protocol

Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

Hybridization protocol

Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Scan protocol

GeneChips were scanned using the Affymetrix GCS 3000.

Description

CD4+CD25+_2

Data processing

The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

Submission date

Mar 29, 2006

Last update date

May 31, 2006

Contact name

Robert Geffers

E-mail(s)

robert.geffers@helmholtz-hzi.de

Phone

+49 531-6181-3058

Organization name

HCI - Helmholtz Centre for Infection Research

Department

Dep. Molecular Bacteriology

Lab

Genome Analytics

Street address

Inhoffenstr. 7

City

Braunschweig

ZIP/Postal code

38124

Country

Germany

Platform ID

GPL96

Series (1)

GSE4571

Expression profiling of Treg cell versus their controls in human and mouse

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	300.4	P	0.000297
AFFX-BioB-M_at	475.9	P	0.000052
AFFX-BioB-3_at	338.7	P	0.000095
AFFX-BioC-5_at	721.1	P	0.000044
AFFX-BioC-3_at	597.9	P	0.000044
AFFX-BioDn-5_at	963.5	P	0.000052
AFFX-BioDn-3_at	4226	P	0.000044
AFFX-CreX-5_at	6817.6	P	0.000044
AFFX-CreX-3_at	11229.9	P	0.000044
AFFX-DapX-5_at	9.2	A	0.48511
AFFX-DapX-M_at	2.5	A	0.645547
AFFX-DapX-3_at	0.9	A	0.993129
AFFX-LysX-5_at	5.9	A	0.455413
AFFX-LysX-M_at	17.4	A	0.559354
AFFX-LysX-3_at	1.4	A	0.966323
AFFX-PheX-5_at	6.4	A	0.686277
AFFX-PheX-M_at	1	A	0.9273
AFFX-PheX-3_at	12.1	A	0.354453
AFFX-ThrX-5_at	3.7	A	0.52976
AFFX-ThrX-M_at	3	A	0.659339

Total number of rows: 22283

Table truncated, full table size 581 Kbytes.

Supplementary data files not provided