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Sample GSM102102

Query DataSets for GSM102102

Status

Public on May 31, 2006

Title

CD4+ CD25-_1 (HG-U133B)

Sample type

RNA

Source name

CD4+CD25-

Organism

Homo sapiens

Characteristics

genetic modification:
tissue: Blood

Growth protocol

ex vivo

Extracted molecule

total RNA

Extraction protocol

Trizol

Label

biotin

Label protocol

Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

Hybridization protocol

Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Scan protocol

GeneChips were scanned using the Affymetrix GCS 3000.

Description

CD4+CD25-_1

Data processing

The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

Submission date

Mar 29, 2006

Last update date

May 31, 2006

Contact name

Robert Geffers

E-mail(s)

robert.geffers@helmholtz-hzi.de

Phone

+49 531-6181-3058

Organization name

HCI - Helmholtz Centre for Infection Research

Department

Dep. Molecular Bacteriology

Lab

Genome Analytics

Street address

Inhoffenstr. 7

City

Braunschweig

ZIP/Postal code

38124

Country

Germany

Platform ID

GPL97

Series (1)

GSE4571

Expression profiling of Treg cell versus their controls in human and mouse

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	835.9	P	0.000225
AFFX-BioB-M_at	1331.5	P	0.000195
AFFX-BioB-3_at	804.4	P	0.001102
AFFX-BioC-5_at	1806.6	P	0.00006
AFFX-BioC-3_at	1877.3	P	0.000044
AFFX-BioDn-5_at	3189.7	P	0.00011
AFFX-BioDn-3_at	10057.3	P	0.000044
AFFX-CreX-5_at	18793.7	P	0.000044
AFFX-CreX-3_at	31611.7	P	0.000044
AFFX-DapX-5_at	33.8	A	0.425962
AFFX-DapX-M_at	14.4	A	0.699394
AFFX-DapX-3_at	7.5	A	0.979978
AFFX-LysX-5_at	81	M	0.054213
AFFX-LysX-M_at	68.6	A	0.340661
AFFX-LysX-3_at	27	A	0.52976
AFFX-PheX-5_at	76.9	A	0.216524
AFFX-PheX-M_at	3.3	A	0.966323
AFFX-PheX-3_at	24.8	A	0.699394
AFFX-ThrX-5_at	59.1	A	0.574038
AFFX-ThrX-M_at	4.7	A	0.945787

Total number of rows: 22645

Table truncated, full table size 581 Kbytes.

Supplementary data files not provided