|
| Status |
Public on Oct 13, 2012 |
| Title |
hsa-miR-590-3p |
| Sample type |
SRA |
| |
|
| Source name |
Neonatal cardiomyocytes
|
| Organism |
Mus musculus |
| Characteristics |
strain: CD1 mice
cell type: cardiomyocytes
age: post natal day 1
treatment: transfected with hsa-miR-590-3p
|
| Treatment protocol |
Transfection of miRNAs mimics was performed at final concentration of 50nM through a reverse transfection protocol using Lipofectamine RNAimax reagent.
|
| Growth protocol |
9.0×105 cardiomyocytes were seeded onto Primaria 60-mm dishes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA, including small RNA fraction was isolated from cardiomyocytes using miRNeasy mini kit (Qiagen), according to manufacturer´s instructions.
Samples were processed using TruSeq RNA-seq sample prep kit from Illumina, according to manufacturer´s instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
mouse cardiomyocytes transfected with hsa-miR-590-3p
|
| Data processing |
Illumina Pipeline 1.8.0 was used for data analysis (Casava v1.8)
Reads Per Kilobase of exon per Megabase of library size (RPKM) were calculated according to Mortazavi, A. et al Nature methods 5, 621-628 (2008).
Genome_build: mm8
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Oct 12, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Miguel Mano |
| E-mail(s) |
mano@icgeb.org
|
| URL |
http://www.icgeb.org/high-throughput-screening.html
|
| Organization name |
International Centre for Genetic Engineering and Biotechnology - ICGEB
|
| Lab |
Molecular Medicine
|
| Street address |
Padriciano 99
|
| City |
Trieste |
| ZIP/Postal code |
34149 |
| Country |
Italy |
| |
|
| Platform ID |
GPL13112 |
| Series (2) |
| GSE41538 |
Transcript expression levels of neonatal mouse cardiomyocytes mock-transfected, or transfected with cel-miR-67, hsa-miR-590-3p or hsa-miR-199a-3p |
| GSE41539 |
Neonatal and adult cardiomyocytes |
|
| Relations |
| SRA |
SRX193720 |
| BioSample |
SAMN01765204 |
