|
| Status |
Public on May 10, 2006 |
| Title |
A172_2 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor cell line A172
|
| Organism |
Homo sapiens |
| Characteristics |
commonly used glioma line
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Standard Affymetrix procedures
|
| Scan protocol |
Standard Affymetrix procedures
|
| Description |
Tumor cell line A172, cultured in serum
|
| Data processing |
Affymetrix GCOS 1.2 with default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Mar 24, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Howard Fine |
| E-mail(s) |
hfine@mail.nih.gov
|
| Phone |
301-402-6383
|
| Fax |
301-480-2246
|
| Organization name |
National Cancer Institute
|
| Department |
Neurological Disorders and Stroke
|
| Street address |
9030 Old Georgetown Rd
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE4536 |
Tumor stem cells more closely mirror the phenotype and genotype of primary human tumors than do cancer cell lines |
|
| Relations |
| Reanalyzed by |
GSE119087 |
