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Sample GSM101519

Query DataSets for GSM101519

Status

Public on May 30, 2006

Title

Treg_1

Sample type

RNA

Source name

CD4+CD25hi

Organism

Homo sapiens

Characteristics

genetic modification:
tissue: T-cells

Growth protocol

stimulated with irradiated allogenic EBV B-cells, IL-2

Extracted molecule

total RNA

Extraction protocol

Qiagen, RNAeasy

Label

biotin

Label protocol

Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).

Hybridization protocol

Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at conditions recommended by the manufacturer. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.

Scan protocol

GeneChips were scanned using the Affymetrix GCS 3000.

Description

Treg_1

Data processing

The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.

Submission date

Mar 23, 2006

Last update date

May 30, 2006

Contact name

Robert Geffers

E-mail(s)

robert.geffers@helmholtz-hzi.de

Phone

+49 531-6181-3058

Organization name

HCI - Helmholtz Centre for Infection Research

Department

Dep. Molecular Bacteriology

Lab

Genome Analytics

Street address

Inhoffenstr. 7

City

Braunschweig

ZIP/Postal code

38124

Country

Germany

Platform ID

GPL96

Series (1)

GSE4527

Ectopic expression of Foxp3 and UBD in Th cells

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	764.5	P	0.00034
AFFX-BioB-M_at	1723.8	P	0.000044
AFFX-BioB-3_at	1146.2	P	0.00006
AFFX-BioC-5_at	1444.7	P	0.00006
AFFX-BioC-3_at	1350.1	P	0.000052
AFFX-BioDn-5_at	2613	P	0.000052
AFFX-BioDn-3_at	9066.2	P	0.000044
AFFX-CreX-5_at	15868.3	P	0.000044
AFFX-CreX-3_at	21615.6	P	0.000044
AFFX-DapX-5_at	24.6	A	0.425962
AFFX-DapX-M_at	40.8	A	0.368438
AFFX-DapX-3_at	8.8	A	0.876428
AFFX-LysX-5_at	18.3	A	0.425962
AFFX-LysX-M_at	49.7	A	0.455413
AFFX-LysX-3_at	39.8	A	0.455413
AFFX-PheX-5_at	4.8	A	0.9273
AFFX-PheX-M_at	6.1	A	0.783476
AFFX-PheX-3_at	73.7	A	0.340661
AFFX-ThrX-5_at	8.1	A	0.783476
AFFX-ThrX-M_at	17.3	A	0.645547

Total number of rows: 22283

Table truncated, full table size 585 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM101519.CEL.gz	3.4 Mb	(ftp)(http)	CEL
Processed data included within Sample table