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| Status |
Public on Sep 13, 2012 |
| Title |
Broad_ChipSeq_A549_H3K27ac_DEX_100nM |
| Sample type |
SRA |
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| Source name |
A549
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| Organism |
Homo sapiens |
| Characteristics |
biomaterial_type: immortalized cell line
line: A549
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: abcam
antibody vendorid: ab4729
controlid: wgEncodeEH003075
replicate: 1,2
softwareversion: ScriptureVPaperR3
cell sex: M
antibody: H3K27ac
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: Abcam
antibody vendorid: ab4729
treatment: DEX_100nM
treatment description: 1 h with 100 nM Dexamethasone (Myers)
control: std
control description: Standard input signal for most experiments.
controlid: A549/DEX_100nM/Input/std
labversion: Illumina_HiSeq_2000
labversion description: Illumina HiSeq 2000
softwareversion: ScriptureVPaperR3
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| Biomaterial provider |
ATCC
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| Treatment protocol |
DEX_100nM
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Instrument model unknown. ("Illumina Genome Analyzer" specified by default). For more information, see http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 2000 |
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| Data processing |
http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=hg19&g=wgEncodeBroadHistone
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| Submission date |
Sep 13, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
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| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
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| Platform ID |
GPL11154 |
| Series (1) |
| GSE29611 |
Histone Modifications by ChIP-seq from ENCODE/Broad Institute |
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| Relations |
| SRA |
SRX186689 |
| BioSample |
SAMN01174105 |
| Named Annotation |
GSM1003493_hg19_wgEncodeBroadHistoneA549H3k27acDex100nmSig.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM1003493_hg19_wgEncodeBroadHistoneA549H3k27acDex100nmPk.broadPeak.gz |
849.4 Kb |
(ftp)(http) |
BROADPEAK |
| GSM1003493_hg19_wgEncodeBroadHistoneA549H3k27acDex100nmSig.bigWig |
400.2 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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