NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1000818 Query DataSets for GSM1000818
Status Public on Sep 10, 2014
Title Eµ-myc Lymphoma Train 29
Sample type RNA
 
Source name Lymphoma
Organism Mus musculus
Characteristics genotype/variation: Eµ-myc
tissue type: lymphoma tumor
Treatment protocol N/A
Growth protocol N/A
Extracted molecule total RNA
Extraction protocol Lymphoma tissue was homogenized with a mortar and pestle and with sonication. RNA was extracted using the Rneasy Mini Kit (Qiagen)
Label biotin
Label protocol Target preparation from total RNA was performed using T7 primers in the cDNA synthesis step, followed by in vitro transcription to produce biotin-labeled cRNA.
 
Hybridization protocol The cRNA was fragmented and mixed into a hybridization cocktail before application to the array, which was then hybridized for 16 hours at 45C with rotation at 60 rpm.
Scan protocol Immediately following hybridization, the arrays underwent an automated washing and staining protocol on the GeneChip FS450 fluidics station and were then scanned on the GeneChip Scanner 3000 7G Plus.
Data processing The data were analyzed with RMA normalization using the standard settings in the affy package from Bioconductor using R.
 
Submission date Sep 10, 2012
Last update date Sep 10, 2014
Contact name Daphne R Friedman
E-mail(s) daphne.friedman@duke.edu
Organization name Duke University Medical Center
Street address 101 Science Drive, Room 2121, CIEMAS Bldg
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
 
Platform ID GPL1261
Series (2)
GSE40756 Transgenic Eµ-myc mouse lymphoma expression data for training dataset
GSE40760 The Eµ-myc Mouse Model Represents Heterogeneity Across Human Aggressive B-cell Lymphomas

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1415670_at 8.981706934
1415671_at 9.967832091
1415672_at 11.6784141
1415673_at 9.503138913
1415674_a_at 9.751663919
1415675_at 9.528400254
1415676_a_at 11.64939113
1415677_at 8.680016836
1415678_at 10.39017031
1415679_at 11.51314096
1415680_at 10.62173393
1415681_at 10.93563519
1415682_at 9.495707332
1415683_at 11.53176885
1415684_at 8.743371729
1415685_at 9.755049895
1415686_at 9.366706796
1415687_a_at 12.52107249
1415688_at 10.47649909
1415689_s_at 9.485893161

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1000818_SMM27.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap