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Sample GSM1000139 Query DataSets for GSM1000139
Status Public on Sep 10, 2012
Title LICR_ChipSeq_MEF_H3K27ac_adult-8wks
Sample type SRA
 
Source name MEF
Organism Mus musculus
Characteristics lab: LICR-m
lab description: Ren - Ludwig Institue for Cancer Research
datatype: ChipSeq
datatype description: Chromatin IP Sequencing
cell: MEF
cell organism: mouse
cell description: Mouse Embryonic Fibroblast
cell sex: M
antibody: H3K27ac
antibody antibodydescription: rabbit polyclonal. Antibody Target: H3K27ac
antibody targetdescription: Histone H3 (acetyl K27). As with H3K9ac, associated with transcriptional initiation and open chromatin structure. It remains unknown whether acetylation has can have different consequences depending on the specific lysine residue targeted. In general, though, there appears to be high redundancy. Histone acetylation is notable for susceptibility to small molecules and drugs that target histone deacetylases.
antibody vendorname: Abcam
antibody vendorid: ab4729
age: adult-8wks
age description: Adult 8 weeks
age: adult-8wks
age description: Adult 8 weeks
control: std
control description: Standard input signal for most experiments.
controlid: wgEncodeEM001456
labversion: Pooled & filtered
softwareversion: MACS
strain: C57BL/6
strain description: C57 black 6, the most common inbred strain of laboratory mouse
Biomaterial provider LICR lab
Growth protocol MEF_Ren_protocol.pdf
Extracted molecule genomic DNA
Extraction protocol http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeLicrHistone
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina HiSeq 2000
 
Data processing http://genome.ucsc.edu/cgi-bin/hgTrackUi?db=mm9&g=wgEncodeLicrHistone
 
Submission date Sep 10, 2012
Last update date May 15, 2019
Contact name ENCODE DCC
E-mail(s) encode-help@lists.stanford.edu
Organization name ENCODE DCC
Street address 300 Pasteur Dr
City Stanford
State/province CA
ZIP/Postal code 94305-5120
Country USA
 
Platform ID GPL13112
Series (3)
GSE31039 Histone Modifications by ChIP-seq from ENCODE/LICR
GSE49847 A comparative encyclopedia of DNA elements in the mouse genome
GSE51334 DNA replication-timing boundaries separate stable chromosome domains with cell-type-specific functions
Relations
SRA SRX185860
BioSample SAMN01163878
Named Annotation GSM1000139_mm9_wgEncodeLicrHistoneMefH3k27acMAdult8wksC57bl6StdSig.bigWig

Supplementary file Size Download File type/resource
GSM1000139_mm9_wgEncodeLicrHistoneMefH3k27acMAdult8wksC57bl6StdPk.broadPeak.gz 593.9 Kb (ftp)(http) BROADPEAK
GSM1000139_mm9_wgEncodeLicrHistoneMefH3k27acMAdult8wksC57bl6StdSig.bigWig 43.9 Mb (ftp)(http) BIGWIG
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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