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| Status |
Public on Sep 20, 2018 |
| Title |
Extensive sex differences at the initiation of meiotic recombination |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
Third-party reanalysis
Methylation profiling by high throughput sequencing
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| Summary |
Meiotic recombination differs between males and females, however, when and how these differences are established is unknown. We identify extensive sex differences at recombination initiation by mapping hotspots of meiotic DNA double strand breaks in male and female mice. Contrary to past findings in humans, few hotspots are used uniquely in either sex. Instead, grossly different recombination landscapes result from up to 15-fold differences in hotspot use between males and females. Indeed, most recombination occurs at sex-biased hotspots. Sex biased hotspots appear to be partly determined by chromosome structure, and DNA methylation, absent in females at the onset of meiosis, plays a substantial role. Sex differences are also evident later in meiosis as the repair frequency of distal meiotic breaks as crossovers diverges in males and females. Suppression of distal crossovers may help to minimize age-related aneuploidy that arises due to cohesion loss during dictyate arrest in females.
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| Overall design |
Comparison of meiotic DNA double strand break hotspots in mouse testis and ovary
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| Contributor(s) |
Brick K, Thibault-Sennett S, Smagulova F, Lam KW, Pu Y, Pratto F, Camerini-Otero RD, Petukhova GV |
| Citation(s) |
30185906 |
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| Submission date |
Jun 12, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Kevin Brick |
| E-mail(s) |
brickkm@mail.nih.gov, kevbrick@gmail.com, brickkm@niddk.nih.gov
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| Organization name |
NIDDK
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| Department |
GBB
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| Street address |
5/205 Memorial Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (3) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA390158 |
| SRA |
SRP109024 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99921_RAW.tar |
61.1 Gb |
(http)(custom) |
TAR (of BAM, BED, BEDGRAPH) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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