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Series GSE99714 Query DataSets for GSE99714
Status Public on Sep 11, 2017
Title Distinct mesenchymal lineages and niches promote epithelial self-renewal and myofibrogenesis in the lung [Single cell RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Single cell RNAseq using the 10x gemcode platform on bulk mesenchyme from one adult 6w old mouse.
Overall design Lungs were harvested from a 6-week old adult mouse and digested to generate a single-cell suspension. The cell suspension was stained with antibodies directed against Epcam, CD31 and CD45. DAPI was included as a live/dead stain. Cells that were negative for DAPI, EPCAM, CD31 and CD45, were sorted into collection buffer. The purified cells, referred to as bulk mesenchyme, were manually counted and subsequently loaded onto the 10x Genomics instrument, libraries were prepared according to the manufacture's protocol.
Contributor(s) Zepp JA, Morley MP, Morrisey EE
Citation(s) 28886382
Submission date Jun 06, 2017
Last update date Jul 25, 2021
Contact name Michael Patrick Morley
Phone 215-898-2026
Organization name Perelman School of Medicine at the University of Pennsylvania
Department Penn Cardiovascular Institute
Street address 3400 Civic Center Blvd, Bldg 421
City Philadelphia
State/province PA
ZIP/Postal code 19104
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (1)
GSM2650814 Bulk lung mesenchyme
BioProject PRJNA389398
SRA SRP108668

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Supplementary file Size Download File type/resource
GSE99714_RAW.tar 23.1 Mb (http)(custom) TAR (of MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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