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| Status |
Public on Sep 11, 2017 |
| Title |
Distinct mesenchymal lineages and niches promote epithelial self-renewal and myofibrogenesis in the lung [Single cell RNA-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single cell RNAseq using the 10x gemcode platform on bulk mesenchyme from one adult 6w old mouse.
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| Overall design |
Lungs were harvested from a 6-week old adult mouse and digested to generate a single-cell suspension. The cell suspension was stained with antibodies directed against Epcam, CD31 and CD45. DAPI was included as a live/dead stain. Cells that were negative for DAPI, EPCAM, CD31 and CD45, were sorted into collection buffer. The purified cells, referred to as bulk mesenchyme, were manually counted and subsequently loaded onto the 10x Genomics instrument, libraries were prepared according to the manufacture's protocol.
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| |
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| Contributor(s) |
Zepp JA, Morley MP, Morrisey EE |
| Citation(s) |
28886382 |
| |
| Submission date |
Jun 06, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Michael Patrick Morley |
| E-mail(s) |
mmorley@pennmedicine.upenn.edu
|
| Phone |
215-898-2026
|
| Organization name |
Perelman School of Medicine at the University of Pennsylvania
|
| Department |
Penn Cardiovascular Institute
|
| Street address |
3400 Civic Center Blvd, Bldg 421
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA389398 |
| SRA |
SRP108668 |
