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| Status |
Public on Jul 05, 2017 |
| Title |
The functional enhancer repertoire of human embryonic stem cells [ChIP-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We combined Self-Transcribing Active Regulatory Region Sequencing (STARR-seq) with an enrichment step using chromatin immunoprecipitation in a massively parallel reporter assay. We applied this assay, termed ChIP-STARR-seq, to normal (primed) and naive human embryonic stem cells, building up a comprehensive catalogue of functional enhancers. This database record describes the ChIP-seq and BAC component.
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| |
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| Overall design |
ChIP-seq for OCT4 (POU5F1), NANOG, H3K27ac, H3K4me1 + Input control in naive and primed hESCs = 14 datasets total + 1 BAC library
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| Contributor(s) |
Barakat TS, Halbritter F, Rendeiro AF, Zhang M, Bock C, Chambers I |
| Citation(s) |
30033119 |
| |
| Submission date |
Jun 02, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Florian Halbritter |
| Organization name |
St. Anna Children's Cancer Research Institute (CCRI)
|
| Lab |
Developmental Cancer Genomics
|
| Street address |
Zimmermannplatz 10
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE99631 |
The functional enhancer repertoire of human embryonic stem cells |
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| Relations |
| BioProject |
PRJNA389113 |
| SRA |
SRP108517 |
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