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Series GSE99622 Query DataSets for GSE99622
Status Public on Jun 19, 2017
Title Sex differences in microglial developmental gene expression programs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary During normal neurodevelopment, microglia perform a range of different functions that are crucial for the proper development, circuit integration, and function of neurons. These functions include certain types of synaptic pruning during critical stages of neurodevelopment (Schafer et al., 2012), and the production of cytokines and chemokines during normal physiological tasks, proteins which are important for homeostasis in addition to immune defense (Avital et al., 2003; Goshen et al., 2007). Microglia and the inflammatory cytokines they produce (e.g., in response to injury or infection) are also increasingly implicated in neural dysfunction during development and the risk of neuropsychiatric disorders. Increasing evidence suggests many neurological disorders emerge because of disrupted neuronal developmental trajectory (Marin, 2016). Considering the critical role of microglia for integration of neurons into their proper circuitry as well in proper synapse formation, there is a pressing need to understand the mechanisms underlying normal and abnormal microglia development, their interactions with specific neuronal subsets, and to understand the utility of these same analyses for human tissue. Moreover, as there are pronounced sex differences in the presentation of many neurological disorders, it is critical to understand these mechanisms in both males and females. Here we study developmental gene expression changes in male and female mice from E18 to P60. We isolated microglia from male and female hippocampi of mice from different developmental ages ranging from embryonic day 18 (E18) to postnatal day 60 (P60). Hippocampus was selected based on its importance to mental health, its marked vulnerability relative to other brain regions in response to diverse threats to homeostasis (epilepsy, stroke, and cardiac arrest (Fujioka et al., 2000; Petito, Feldmann, Pulsinelli, & Plum, 1987; Salmenpera, Kalviainen, Partanen, & Pitkanen, 1998; Sapolsky, Uno, Rebert, & Finch, 1990)), and due to evidence of morphological sex differences in microglial development in this region (Schwarz, Sholar, & Bilbo, 2012). It was found that developmental gene expression changes were more advanced in females at P60 and that treatment with LPS, a potent immune activator, could normalize developmental gene expression differences in males.
Overall design developmental timecourse in males and females; LPS vs. Saline at P60 in males and females
Contributor(s) Alter M, Bilbo S
Citation(s) 28618077
Submission date Jun 02, 2017
Last update date May 15, 2019
Contact name mark alter
Organization name University of Pennsylvania
Department Center for Neurobiology and Behvior
Street address 125 S. 31st Street
City Philadelphia
State/province PA
ZIP/Postal code 19003
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (60)
GSM2648766 F_E18 1
GSM2648767 M_E18 1
GSM2648768 F_E18 3
BioProject PRJNA388977
SRA SRP108497

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Supplementary file Size Download File type/resource
GSE99622_TranscriptRawReadCounts.xls.gz 7.1 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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