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| Status |
Public on Jul 12, 2017 |
| Title |
Identification of LEC1 binding sites in Arabidopsis seedlings |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
LEAFY COTYLEDON1 (LEC1), an atypical subunit of the NF-Y CCAAT binding transcription factor, is a central regulator that controls many aspects of seed development including the maturation phase during which seeds accumulate storage macromolecules and embryos acquire the ability to withstand desiccation. To define the gene network and developmental processes controlled by LEC1, genes regulated directly by and downstream of LEC1 were identified. In this part of the study, we identified the genes bound by LEC1 by overexpressing a FLAG-tagged inducible form of LEC1 in Arabidopsis seedlings and inducing LEC1 activity for a short (4 hours) or long (8 days) period of time, to identify the genes bound EARLY and LATE, respectively.
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| Overall design |
LEC1 activity was induced in Arabidopsis 35S:FLAG-LEC1-GR plants, either by treating seedlings for 4h, or germinating seeds and growing seedlings for 8 days in the presence of dexamethasone (DEX). Anti-FLAG antibodies (Sigma F1804) were used to immunoprecipitate LEC1-bound DNA fragments and compared to total fragmented chromatin from the same biological materials. Two biological replicates per conditions were analyzed.
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| Contributor(s) |
Pelletier J, Kwong RW, Bui AQ, Le BH, Golberg RB, Harada JJ |
| Citation(s) |
28739919 |
| Submission date |
May 31, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
John Harada |
| E-mail(s) |
jjharada@ucdavis.edu
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| Organization name |
University of California
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| Department |
Plant Biology
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| Lab |
Harada lab
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| Street address |
One Shields Avenue
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| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
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| Platforms (1) |
| GPL10977 |
[At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA388705 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE99529_FL1GR_4hD_peaks.bed.gz |
24.6 Kb |
(ftp)(http) |
BED |
| GSE99529_FL1GR_8dD_peaks.bed.gz |
37.7 Kb |
(ftp)(http) |
BED |
| GSE99529_RAW.tar |
418.1 Mb |
(http)(custom) |
TAR (of BAR, CEL) |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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