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Series GSE99528 Query DataSets for GSE99528
Status Public on Jul 12, 2017
Title mRNA changes following induction of LEC1 activity in Arabidopsis seedlings
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary LEAFY COTYLEDON1 (LEC1), an atypical subunit of the NF-Y CCAAT binding transcription factor, is a central regulator that controls many aspects of seed development including the maturation phase during which seeds accumulate storage macromolecules and embryos acquire the ability to withstand desiccation. To define the gene network and developmental processes controlled by LEC1, genes regulated directly by and downstream of LEC1 were identified. In this part of the study, we identified the genes regulated by LEC1 by overexpressing an inducible form of LEC1 in Arabidopsis seedlings and identifying the mRNAs induced EARLY (1h) after induction of LEC1 activity.
Overall design Shoot apices from Arabidopsis thaliana 35S:LEC1-GR 8 day old seedlings were incubated for 1 h in the presence or absence of Dexamethasone to induce LEC1 activity. Two biological replicates per treatment were analyzed.
Contributor(s) Pelletier J, Kwong RW, Bui AQ, Le BH, Golberg RB, Harada JJ
Citation(s) 28739919
Submission date May 31, 2017
Last update date Jul 25, 2021
Contact name John Harada
Organization name University of California
Department Plant Biology
Lab Harada lab
Street address One Shields Avenue
City Davis
State/province CA
ZIP/Postal code 95616
Country USA
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (4)
GSM2645483 LEC1GR NoDEX br1
GSM2645484 LEC1GR NoDEX br2
GSM2645485 LEC1GR DEX br1
BioProject PRJNA388706

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99528_RAW.tar 12.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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