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Series GSE99479 Query DataSets for GSE99479
Status Public on Oct 16, 2017
Title A class of GATA3 mutation reprograms the breast cancer transcriptional network through gain and loss of function
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary A pioneer transcription factor, GATA3, is one of the most frequently mutated genes in breast cancer, yet the impact of these mutations is largely unknown. We generated a GATA3 mutant cell line (T47D wt/R330fs) by CRISPR. Mutation of one allele of GATA3 led to loss of binding and decreased expression at a subset of genes, including Progesterone Receptor. At other loci, associated with epithelial to mesenchymal transition, gain of binding at a novel sequence motif correlated with increased gene expression. Our results illuminate tumor-promoting functions of specific GATA3 mutations in breast cancer.
Overall design Genome-wide mapping of chromatin localization of luminal transcription factors in GATA3 mutant cells
Contributor(s) Takaku M, Grimm SA, Paul WA
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Submission date May 31, 2017
Last update date May 15, 2019
Contact name Paul A Wade
Phone 919-541-3392
Organization name NIEHS
Department Laboratory of Molecular Carcinogenesis
Street address 111 TW Alexander Drive
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (96)
GSM2644561 ATAC-seq_T47Dflp_rep1
GSM2644562 ATAC-seq_T47Dflp_rep2
GSM2644563 ATAC-seq_T47Dflp_rep3
BioProject PRJNA388581
SRA SRP108337

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Supplementary file Size Download File type/resource
GSE99479_Gene_counts_per_sample.Xenograft_CRctrl.txt.gz 897.9 Kb (ftp)(http) TXT
GSE99479_RNA-seq_DEGs.T47Dflp.vs.CR3flp.txt.gz 24.3 Kb (ftp)(http) TXT
GSE99479_RNA-seq_DEGs.Vector.vs.ExoR330fs.txt.gz 11.2 Kb (ftp)(http) TXT
GSE99479_RNA-seq_DEGs.progesterone.txt.gz 67.9 Kb (ftp)(http) TXT
GSE99479_bed_files.tar.gz 3.3 Mb (ftp)(http) TAR
GSE99479_bigwig_files.tar.gz 10.5 Gb (ftp)(http) TAR
GSE99479_gene_counts_per_sample.RNA-seq.txt.gz 1.0 Mb (ftp)(http) TXT
GSE99479_gene_counts_per_sample.RNA-seq_ProgesteroneTreatment.txt.gz 873.1 Kb (ftp)(http) TXT
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Processed data are available on Series record

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