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| Status |
Public on Jun 01, 2017 |
| Title |
SNP genotype data from induced Pluripotent Stem cells from Parkinson's Disease patients harboring mutations in the GBA1 gene, and from healthy control donors |
| Organism |
Homo sapiens |
| Experiment type |
SNP genotyping by SNP array
Genome variation profiling by SNP array
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| Summary |
We have generated human induced Pluripotent Stem cells (hiPSc) using Retroviral and Sendai virus-mediated delivery of reprogramming factors. hiPSc lines have been screened using SNP array to assess chromosomal stability (alongside the fibroblast lines from which they were derived), and validation of the pluripotency of the hiPSc lines is provided by Pluritest assessment of transcriptome datasets, prior to differentiation and downstream assays.
Proteomic analysis of GBA1 patient-derived dopaminergic neurons reveal impeded neurite outgrowth.
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| Overall design |
human iPSc lines were derived from human dermal fibroblasts from PD patients harboring the mutation N370S in GBA1, and control donors. SNP datasets were generated from 7 previously unpublished iPSC clones and the fibroblasts from which they were derived
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| Contributor(s) |
Bogetofte H, Ryan B, Jensen P, Vergoossen D, Caffrey T, Kiani L, Fernandez H, Vowles J, Meyer M, Cowley SA, Røssel-Larsen M, Wade-Martins R |
| Citation missing |
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| |
| Submission date |
May 31, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
sally.cowley@path.ox.ac.uk
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| Phone |
+44 (0) 1865 275600
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| Organization name |
University of Oxford
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| Department |
Sir William Dunn School of Pathology
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| Lab |
James Martin Stem Cell Facility
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| Street address |
South Parks Road
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| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL13829 |
Illumina HumanCytoSNP-12 v2.1 BeadChip |
| GPL22678 |
HumanOmniExpress-24 v1.1 BeadChip [SNP_ID version] |
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| Samples (13)
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| This SubSeries is part of SuperSeries: |
| GSE99473 |
SNP Genotype and Gene Expression data from induced Pluripotent Stem cells from Parkinson's Disease patients harboring mutations in the GBA1 gene, and from healthy control donors |
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| Relations |
| BioProject |
PRJNA388560 |
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