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Series GSE99402 Query DataSets for GSE99402
Status Public on Dec 06, 2017
Title ChIP-RX of GM15850 cells treated with synthetic transcription elongation factors
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Switching a paused RNA polymerase II into productive elongation is tightly-regulated, especially at genes involved in human development and disease. To exert control on this rate-limiting step, we designed sequence-specific synthetic transcription elongation factors (Syn-TEFs). These molecules are composed of programmable DNA-binding ligands flexibly tethered to a small molecule that binds a component of the transcription elongation machinery. The resultant bifunctional molecules convert constituent modules from broad-spectrum inhibitors of transcription into a gene-specific stimulator of transcriptional elongation. Here, we present Syn-TEF1, a molecule that actively facilitates transcription across repressive GAA repeats that silence frataxin expression in Friedreich’s ataxia, a debilitating and ultimately lethal neurodegenerative disease with no effective therapy.
 
Overall design The global effect on expression of synthetic transcription elongation factor (Syn-TEF1) and its constituent parts was examined in cells from a patient with FRDA (GM15850) and a healthy sibling control patient (GM15851). Spike-ins from mouse (mm9) were used for normalization.
 
Contributor(s) Erwin GS, Grieshop MP, Ali A, Lawlor MA, Ansari AZ
Citation(s) 29192133
Submission date May 30, 2017
Last update date May 15, 2019
Contact name Aseem Ansari
Organization name University of Wisconsin-Madison
Department Biochemistry and The Genome Center
Lab Ansari
Street address 440 Henry Mall
City Madison
State/province WI
ZIP/Postal code 53706
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (18)
GSM2643536 BRD4_Input_1000nM_PA1+JQ1
GSM2643537 BRD4_Input_1000nM_Syn-TEF1
GSM2643538 BRD4_Input_DMSO
This SubSeries is part of SuperSeries:
GSE99403 Synthetic transcription elongation factors
Relations
BioProject PRJNA388395
SRA SRP108252

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