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| Status |
Public on Dec 06, 2017 |
| Title |
RNA-seq of GM15850 and GM15851 cells treated with synthetic transcription elongation factors. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Switching a paused RNA polymerase II into productive elongation is tightly-regulated, especially at genes involved in human development and disease. To exert control on this rate-limiting step, we designed sequence-specific synthetic transcription elongation factors (Syn-TEFs). These molecules are composed of programmable DNA-binding ligands flexibly tethered to a small molecule that binds a component of the transcription elongation machinery. The resultant bifunctional molecules convert constituent modules from broad-spectrum inhibitors of transcription into a gene-specific stimulator of transcriptional elongation. Here, we present Syn-TEF1, a molecule that actively facilitates transcription across repressive GAA repeats that silence frataxin expression in Friedreich’s ataxia, a debilitating and ultimately lethal neurodegenerative disease with no effective therapy.
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| Overall design |
The global effect on expression of synthetic transcription elongation factor (Syn-TEF1) and its constituent parts was examined in cells from a patient with FRDA (GM15850) and a healthy sibling control patient (GM15851).
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| Contributor(s) |
Erwin GS, Grieshop MP, Ali A, Ansari AZ |
| Citation(s) |
29192133 |
| |
| Submission date |
May 30, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Aseem Ansari |
| Organization name |
University of Wisconsin-Madison
|
| Department |
Biochemistry and The Genome Center
|
| Lab |
Ansari
|
| Street address |
440 Henry Mall
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (40)
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| This SubSeries is part of SuperSeries: |
| GSE99403 |
Synthetic transcription elongation factors |
|
| Relations |
| BioProject |
PRJNA388394 |
| SRA |
SRP108251 |
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