|
| Status |
Public on Feb 14, 2018 |
| Title |
Single-cell RNA-seq following APE1/Ref-1 knockdown in Pancreatic Ductal Adenocarcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
APE1 was knocked down using siRNA in low passage patient-derived PDAC cells and the resulting cells, along with control cells were analysed using scRNA-seq to identify differentially expressed genes and pathways as a result of APE1 knock-down.
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| |
|
| Overall design |
siRNA APE1 knock-down versus scrambled control, The SMARTer system was used to generate cDNA from 96 captured single cells. Unstranded 2x100bp reads were sequenced using a HiSeq2500 on rapid run mode in 1 lane.
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| |
|
| Contributor(s) |
Shah F, Goossens E, Atallah NM, Grimard M, Kelley MR, Fishel ML |
| Citation(s) |
28922540, 33658640, 34376225 |
| |
| Submission date |
May 25, 2017 |
| Last update date |
Aug 18, 2021 |
| Contact name |
Nadia Marie Atallah |
| Organization name |
Purdue University
|
| Department |
Center for Cancer Research
|
| Street address |
201 S. University St
|
| City |
West Lafayette |
| State/province |
IN |
| ZIP/Postal code |
47907 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
| Samples (96)
|
|
| Relations |
| BioProject |
PRJNA388083 |
| SRA |
SRP108039 |
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