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Series GSE99302 Query DataSets for GSE99302
Status Public on Aug 31, 2018
Title Subtle asymmetry of gene expression in embryonic and foetal human brains
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Note: this project is a combination of the data deposited here in GEO and data made available by HDBR in ArrayExpress under E-MTAB-4840. Summary: Brain asymmetry in humans, both structurally and functionally, is observed very early during development, and is important for healthy development. Using RNA sequencing, we investigated gene expression profiles of left and right sides of various brain structures in human foetuses aged 5 to 13 weeks post conception. All brain structures showed significant differences between the expression profiles of left and right sides. The differences partly correlated with gene expression changes with age, suggesting left/right differences in maturation rate. In particular on the faster side (right for cerebral cortex, left for all other investigated tissues), different tissues were lateralised for tissue-specific processes. In the youngest forebrain (5.5 weeks), KCTD12, SNAI1 and GATA1 were significantly right-lateralised. SNAI1 is involved in visceral asymmetry in vertebrates, and KCTD12 is important in lateralisation of fish brains. In the youngest midbrain, SOX1 was significantly left-lateralised.
 
Overall design For the part of the project for which data are deposited here, six human embryos in Carnegie stage 15 or 16 were studied. Left and right side of the midbrain and left and right side of the forebrain were dissected, and RNA sequenced. Differential expression analysis per tissue to find left/right differences (paired analysis by embryo).
 
Contributor(s) de Kovel CG, Lisgo SN, Fisher SE, Francks C
Citation(s) 30181561, 30179233
Submission date May 25, 2017
Last update date May 15, 2019
Contact name Clyde Francks
E-mail(s) clyde.francks@mpi.nl
Organization name Max Planck Institute for Psycholinguistics
Department Language & Genetics
Lab asymmetry
Street address Wundtlaan 1
City Nijmegen
ZIP/Postal code 6525 XD
Country Netherlands
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (20)
GSM2640755 S13048 left forebrain
GSM2640756 S13048 left midbrain
GSM2640757 S13048 right forebrain
Relations
BioProject PRJNA388077
SRA SRP108035

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99302_BGI_2Tissues_expression_log2.txt.gz 2.1 Mb (ftp)(http) TXT
GSE99302_RAW.tar 4.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data is available on Series record

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