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Series GSE99185 Query DataSets for GSE99185
Status Public on Dec 19, 2017
Title Role of Sox2 in preimplantation and postimplantation pluripotency
Organism Mus musculus
Experiment type Expression profiling by array
Summary Sox2 is a key transcription factor directing embryonic stem cell (ESC) pluripotency. However, the role of SoxB1 proteins in epiblast stem cell (EpiSC) pluripotency is unknown. Here we compare the transcriptomes of pluripotent cells cultured in different conditions that express different levels of Sox2. While highest Sox2 level in ESCs are enriched for naive pluripotency markers, ESCs and EpiSCs expressing the same Sox2 level were transcriptionally distinct. Thus Sox2 levels do not dictate the distinction between ESC and EpiSC states.
Overall design Microarray gene expression analysis of mouse embryonic stem cells in different culture conditions. 4 conditions: AFN=Activin/FGF/N2B27 (unsorted (All) or sorted by Sox2 expression into High or Low), PCLN=LIF/2i/N2B27, LBN=LIF/BMP/N2B27 (sorted by Sox2 expression into High and Low) and FCSL=LIF/FCS/GMEM (sorted by Sox2 expression into High and Low). 3 replicates per group. 24 samples total.
Contributor(s) Corsinotti A, Wong FC, Tatar T, Szczerbinska I, Halbritter F, Colby D, Gogolok S, Pantier R, Liggat K, Mirfazeli ES, Hall-Ponsele E, Mullin NP, Wilson V, Chambers I
Citation(s) 29256862
Submission date May 22, 2017
Last update date Jul 25, 2021
Contact name Florian Halbritter
Organization name St. Anna Children's Cancer Research Institute (CCRI)
Lab Developmental Cancer Genomics
Street address Zimmermannplatz 10
City Vienna
ZIP/Postal code 1090
Country Austria
Platforms (1)
GPL6887 Illumina MouseWG-6 v2.0 expression beadchip
Samples (24)
GSM2635348 Activin/FGF Hi 1
GSM2635349 Activin/FGF Lo 1
GSM2635350 LIF/BMP Hi 2
BioProject PRJNA387485

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99185_RAW.tar 15.8 Mb (http)(custom) TAR
GSE99185_non_normalized.txt.gz 6.8 Mb (ftp)(http) TXT
Processed data included within Sample table

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