NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE99171 Query DataSets for GSE99171
Status Public on May 15, 2018
Title Selectively targeting bromodomain and extraterminal proteins for degradation as a novel anti-glioblastoma strategy [ChIP-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Purpose: Characterization of mechanism and vulnerability of BET protein dependency in GBM cells. Methods: ChIP-seq and RNA-seq were performed on GBM cells at different time points following treatment with dBET6, a novel BET protein degrader. The transcriptome responses of parental and JQ1-resistant U87 cells to JQ1 and dBET6 were compared as well. Result: This study reveals crucial functions of BET proteins and provides the rationale and therapeutic merits of targeted degradation of BET proteins by dBET6 in GBM.
 
Overall design ChIP-seq of GBM cells following treatment with or without dBET6.
 
Contributor(s) Xu L, Chen Y, Mayakonda A, Koeffler HP
Citation(s) 29764999
Submission date May 22, 2017
Last update date May 15, 2019
Contact name Phillip H Koeffler
E-mail(s) phillip_koeffler@nuhs.edu.sg
Organization name Cancer Science Institute
Lab H. Phillip Koeffler's lab
Street address 14 Medical Drive
City Singapore
ZIP/Postal code 117599
Country Singapore
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (15)
GSM2634750 U87_BRD2
GSM2634751 U87_BRD2_dBET6_500nM_24h
GSM2634752 U87_BRD2_dBET6_500nM_2h
This SubSeries is part of SuperSeries:
GSE99183 Selectively targeting bromodomain and extraterminal proteins for degradation as a novel anti-glioblastoma strategy NA-seq (time course) May 15, 2018 approved TSV
Relations
BioProject PRJNA387463
SRA SRP107788

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99171_RAW.tar 3.6 Gb (http)(custom) TAR (of BW, NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap