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Series GSE99068 Query DataSets for GSE99068
Status Public on Jul 05, 2017
Title IL-6/Stat3-Dependent Induction of Distinct, Obesity-Associated Natural Killer Cells Deteriorates Energy and Glucose Homeostasis
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Natural killer (NK) cells contribute to the development of obesity-associated insulin resistance. We demonstrate that in mice obesity promotes the expansion of interleukin-6 receptor (IL6Ra)-expressing NK cells, which also express a number of other myeloid lineage genes such as the colony-stimulating-factor 1 receptor (Csf1r). Selective ablation of Csf1r- expressing NK cells prevents obesity and insulin resistance. Moreover, conditional inactivation of IL6Ra or Stat3 in NK cells limits obesity-associated formation of myeloid signature NK cells, protects from obesity, insulin resistance and obesity-associated inflammation. Also in humans IL6Ra+ NK cells increase in obesity, correlate with markers of systemic low-grade inflammation and their gene expression profile overlaps with characteristic gene sets of NK cells in obese mice. Collectively, we demonstrate that obesity-associated inflammation and metabolic disturbances depend on IL-6/Stat3-dependent formation of distinct NK cells, which may provide a novel target for the treatment of obesity, metaflammation-associated pathologies and diabetes.
 
Overall design RNA sequencing of two types of NK cells from mouse and human (IL6Ra negative NK cells vs. IL6Ra positive NK cells) and mouse organs (IL6Ra_NKdel vs. IL6Ra_NKflox)
 
Contributor(s) Theurich S, Tsaousidou E, Hanssen R, Lempradl AM, Mauer J, Timper K, Schilbach K, Folz-Donahue K, Heilinger C, Sexl V, Pospisilik JA, Wunderlich FT, Brüning JC
Citation(s) 28683285
Submission date May 18, 2017
Last update date Mar 22, 2019
Contact name Sebastian Theurich
E-mail sebastian.theurich@uk-koeln.de
Organization name Max-Planck-Institute for Metabolism Research and University Hospital of Cologne
Lab Neuronal Control of Metabolism (Brüning Lab)
Street address Gleueler Str. 50
City Köln (Cologne)
ZIP/Postal code 50937
Country Germany
 
Platforms (3)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (63)
GSM2631758 hu_obese NK IL6Ra+ replicate 1
GSM2631759 hu_obese NK IL6Ra- replicate 1
GSM2631760 hu_obese NK IL6Ra- replicate 3
Relations
BioProject PRJNA387175
SRA SRP107327

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE99068_FPKM_RUN1666.xls.gz 5.7 Mb (ftp)(http) XLS
GSE99068_FPKM_RUN1675.xls.gz 3.9 Mb (ftp)(http) XLS
GSE99068_FPKM_RUN1861.xlsx.gz 3.5 Mb (ftp)(http) XLSX
GSE99068_all_cells_mouse_mm10.txt.gz 1.0 Mb (ftp)(http) TXT
GSE99068_celseq_barcodes.192.txt.gz 909 b (ftp)(http) TXT
Raw data are available in SRA
Processed data is available on Series record

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