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Series GSE98969 Query DataSets for GSE98969
Status Public on Jun 12, 2017
Title Single cell RNA-seq identifies a unique microglia type associated with Alzheimer’s disease [RNA]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Alzheimer's disease (AD) is a detrimental neurodegenerative disease with no effective treatments. Due to cellular heterogeneity, the roles of immune cell subsets in AD onset and progression are poorly understood. By transcriptional single cell sorting, we comprehensively map all immune populations in wild type and AD–transgenic (Tg-AD) mouse brains. We describe a novel microglia type associated with neurodegenerative diseases (DAM) and identify the markers, spatial-location, and pathways associated with these cells. Immunohistochemical staining of mice and human brain slices showed DAM with intracellular/phagocytic Aβ particles. Single cell analysis of DAM in Tg-AD and Trem2-/- Tg-AD revealed that the DAM program is activated in a two-step process. Activation is initiated in a Trem2 independent manner which involves down-regulation of microglia checkpoints, followed by activation of a Trem2-dependent program. These data identify a unique microglia-type, which may have important implications for future treatment of AD and other neurodegenerative diseases.
 
Overall design Transcriptional profiling of single cells from immune populations of mouse models of neurodegenerative diseases with matched controls, generated from deep sequencing of tens of thousands of cells, sequenced in several batches on illumina Nextseq500
 
Contributor(s) Amit I, Keren-Shaul H, Spinrad A, Weiner A, Matcovitch-Natan O
Citation(s) 28602351
Submission date May 16, 2017
Last update date Mar 22, 2019
Contact name Ido Amit
E-mail ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (97)
GSM2629341 AB1442
GSM2629342 AB1443
GSM2629343 AB1444
This SubSeries is part of SuperSeries:
GSE98971 Single cell RNA-seq identifies a unique microglia type associated with Alzheimer’s disease
Relations
BioProject PRJNA387079
SRA SRP107339

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98969_RAW.tar 49.9 Mb (http)(custom) TAR (of TXT)
GSE98969_experimental_design_f.txt.gz 382.3 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data provided as supplementary file

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