|
| Status |
Public on May 11, 2017 |
| Title |
A practical solution for preserving single cells for RNA sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We present a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant's Reagent, to stabilise cell samples for single-cell transcriptomic applications.
|
| |
|
| Overall design |
Single-cell RNAseq from 2,3 and 7 day old DSP fixed cells were compared with RNAseq from fresh cells
|
| |
|
| Contributor(s) |
Sharma E, Attar M, Bowden R |
| Citation(s) |
29391536 |
| |
| Submission date |
May 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Eshita Sharma |
| Organization name |
Wellcome Trust Centre for Human Genetics
|
| Department |
Bioinformatics
|
| Street address |
Roosevelt Drive, Headington
|
| City |
Oxford |
| State/province |
Oxfordshire |
| ZIP/Postal code |
OX3 7BN |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (267)
|
|
| Relations |
| BioProject |
PRJNA386155 |
| SRA |
SRP106788 |
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