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Series GSE98527 Query DataSets for GSE98527
Status Public on May 05, 2017
Title Mouse lung transcriptome response to infection with H1N1, H3N2, and H5N1 influenza virus.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The cellular transcriptome of C57BL/6 mouse lungs was profiled by mRNA-Seq analysis at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53. A/Wyoming/03/03 (H3N2) influenza virus replicates poorly in mice and lung tissue collected from mice inoculated with this virus did not carry viral loads detectable by plaque assay.
 
Overall design Six to eight week-old female C57BL/6 mice were infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), or A/Vietnam/1203/04 (H5N1) HALo virus. Lungs from 3 infected mice per experimental group were collected at 12h, 1d, 2d, 3d and 4d post infection. Lungs of mice treated with PBS, collected after 4 d, served as mock control.
 
Contributor(s) Metreveli G, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A
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NIH grant(s)
Grant ID Grant title Affiliation Name
U19 AI106754 Multiscale analysis of influenza host-pathogen interactions: Fluomics MOUNT SINAI SCHOOL OF MEDICINE Adolfo Garcia-Sastre
Submission date May 03, 2017
Last update date Mar 21, 2019
Contact name Max Chang
E-mail mchang@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (48)
GSM2598235 C57BL/6-116_H1N1_PFU1E4_12h_1
GSM2598236 C57BL/6-117_H1N1_PFU1E4_12h_2
GSM2598237 C57BL/6-118_H1N1_PFU1E4_12h_3
Relations
BioProject PRJNA385346
SRA SRP106381

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98527_Mouse_lung_FPKM.csv.gz 7.7 Mb (ftp)(http) CSV
Raw data are available in SRA
Processed data is available on Series record

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