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Series GSE98310 Query DataSets for GSE98310
Status Public on Apr 28, 2017
Title Transcriptome analysis of G protein-coupled receptors in distinct genetic subgroups of acute myeloid leukemia: identification of potential disease-specific targets
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Acute myeloid leukemia (AML) is associated with poor clinical outcome and the development of more effective therapies is urgently needed. G protein-coupled receptors (GPCRs) represent attractive therapeutic targets, accounting for approximately 30% of all targets of marketed drugs. Using next-generation sequencing, we studied the expression of 772 GPCRs in 148 genetically diverse AML specimens, normal blood and bone marrow cell populations as well as cord blood-derived CD34-positive cells. Among these receptors, 30 are overexpressed and 19 are downregulated in AML samples compared with normal CD34-positive cells. Upregulated GPCRs are enriched in chemokine (CCR1, CXCR4, CCR2, CX3CR1, CCR7 and CCRL2), adhesion (CD97, EMR1, EMR2 and GPR114) and purine (including P2RY2 and P2RY13) receptor subfamilies. The downregulated receptors include adhesion GPCRs, such as LPHN1, GPR125, GPR56, CELSR3 and GPR126, protease-activated receptors (F2R and F2RL1) and the Frizzled family receptors SMO and FZD6. Interestingly, specific deregulation was observed in genetically distinct subgroups of AML, thereby identifying different potential therapeutic targets in these frequent AML subgroups.
 
Overall design Total healthy bone marrow was sorted to isolate distinct cell populations. RNA-Seq analysis was performed on sorted cells to determine gene expression profile of healthy bona marrow subpopulations.
 
Contributor(s) Maiga A, Lemieux S, Pabst C, Lavallée V, Bouvier M, Sauvageau G, Hébert J
Citation(s) 27258612
Submission date Apr 27, 2017
Last update date Mar 20, 2019
Contact name Guy Sauvageau
Organization name Institute for Research in Immunology and Cancer
Street address 2950, Chemin de Polytechnique
City Montreal
State/province QC
ZIP/Postal code H3T 1J4
Country Canada
 
Platforms (1)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (22)
GSM2590766 Ery1_II
GSM2590767 Ery2_II
GSM2590768 Ery4_II
Relations
BioProject PRJNA384650
SRA SRP105369

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98310_RAW.tar 10.0 Mb (http)(custom) TAR (of TXT)
Raw data are available in SRA
Processed data provided as supplementary file

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