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Series GSE9814 Query DataSets for GSE9814
Status Public on Dec 28, 2007
Title RpoS regulation of gene expression during exponential growth of E. coli K-12
Organism Escherichia coli K-12
Experiment type Expression profiling by array
Summary This study is to identify the RpoS regulon in MG1655 in early exponential growth. RNA samples from wild type or rpoS mutants were extracted using acidic hot phenol method and hybridized to Affymetrix E. coli Antisense Genome Array.
Keywords: Define regulon of RpoS
 
Overall design A precise rpoS deletion mutant of MG1655 was constructed using the red recombinase method. Wild type and rpoS mutants were inoculated in triplicate into LB media at a starting OD of 0.0001 and grown aerobically at 37C. Cultures were harvested at an OD600 = 0.3 after being maintained in exponential phase for at least eight generations. For RNA extraction, cultures were mixed directly with a boiling lysis buffer containing SDS and EDTA followed by acidic hot phenol (65C) to minimize RNA degradation. RNA samples were hybridized to Affymetrix E. coli Antisense Genome Array according to Affymetrix's standard protocols.
 
Contributor(s) Dong T, Kirchhof MG, Schellhorn HE
Citation(s) 18158608
Submission date Dec 06, 2007
Last update date Sep 20, 2012
Contact name Herb E Schellhorn
E-mail(s) schell@mcmaster.ca
Phone 9055259140-27316
Organization name McMaster University
Department Biology
Lab 405
Street address 1280 Main St. W.,
City Hamilton
State/province ON
ZIP/Postal code L8S4K1
Country Canada
 
Platforms (1)
GPL199 [Ecoli_ASv2] Affymetrix E. coli Antisense Genome Array
Samples (6)
GSM247608 Exp_WT_rep1
GSM247612 Exp_WT_rep2
GSM247613 Exp_WT_rep3
Relations
BioProject PRJNA103767

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9814_RAW.tar 32.1 Mb (http)(custom) TAR (of CEL, CHP)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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