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| Status |
Public on Mar 26, 2018 |
| Title |
ChIP-Seq analysis of transcription factors CREB and C/EBP beta in vasopressin-responsive mouse renal collecting duct mpkCCD cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. Vasopressin binds a G protein-coupled receptor (V2R) activating a cyclic AMP/protein kinase A (PKA) signaling pathway. Sequentially, after activation of cAMP/PKA signaling, many of transcription factors involve gene transcription process. cAMP-response element binding protein (CREB) and cAMP-responsive transcription factor C/EBP beta are potential candidates for vaopressin-mediated regulation of Aqp2 gene transcription proviously reported. In the present study, genome-wide binding sites for two b-ZIP transcription factors CREB and C/EBP beta were identified in vasopressin-responsive mouse collecting duct mpkCCD cells using ChIP-Seq.
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| Overall design |
To identify binding sites of transcription factors CREB and C/EBP beta in cultured mouse collecting duct mpkCCD cells, we carried out ChIP-seq for genome-wide distribution of CREB (n=4) and C/EBP beta (n=2). Observations were made both after 30 minutes treatment with the vasopressin analog dDAVP or vehicle.
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| Contributor(s) |
Jung H, Lee J, Knepper MA |
| Citation(s) |
29572403 |
| |
| Submission date |
Apr 21, 2017 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Hyun Jun Jung |
| E-mail(s) |
hjung24@jhmi.edu
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| Organization name |
Johns Hopkins University School of Medicine
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| Department |
Medicine (Nephrology)
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| Street address |
720 Rutland Ave, Ross Research Building 1165B
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA383865 |
| SRA |
SRP104762 |
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