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Series GSE98051 Query DataSets for GSE98051
Status Public on Jun 19, 2018
Title Evaluation of functional properties of current and novel protein sources using enteroids
Organism Mus musculus
Experiment type Expression profiling by array
Summary This study was designed to address key questions concerning the use of alternative protein sources for animal feeds and addresses aspects such as their nutrient composition and impact on gut function. We used casein (CAS), spray dried porcine plasma (SDPP), soybean meal (SBM), and yellow meal worm (YMW) as protein sources.
We have investigated the use of intestinal organoids as a model to test the effects of different protein sources on the intestinal epithelium. Mouse enteroids were exposed to different undigested protein sources (4% w/v, viz. soybean meal, SBM; casein, CAS; spray dried plasma protein, SDPP; and yellow meal worm, YMW) or DMEM as a control. Microarrays were used to detail the global gene expression.
 
Overall design Confluent 2D monolayers generated from mouse 3D enteroids derived of the duodenum were exposed to different undigested protein sources (4% w/v, viz. soybean meal, SBM; casein, CAS; spray dried plasma protein, SDPP; and yellow meal worm, YMW) or DMEM as a control and RNA isolated for genome-wide transcriptomics.
 
Contributor(s) Kar SK, Boekschoten M
Citation(s) 32467755
Submission date Apr 21, 2017
Last update date Jul 25, 2021
Contact name Soumya Kanti Kar
E-mail(s) soumya.kar@wur.nl
Organization name WUR
Department Animal Science Group
Lab Host Microbe Interactomics
Street address De Elst1
City Wageningen
State/province I am not in the U.S. or Canada
ZIP/Postal code 6708WD
Country Netherlands
 
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (15)
GSM2586168 SBM, biological rep1
GSM2586169 CAS, biological rep1
GSM2586170 SDPP, biological rep1
Relations
BioProject PRJNA383808

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Supplementary file Size Download File type/resource
GSE98051_RAW.tar 66.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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