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Series GSE98048 Query DataSets for GSE98048
Status Public on Apr 22, 2017
Title Single-cell profiling of tumor infiltrating T cells and macrophages [RNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Effective therapies for non-small cell lung cancer (NSCLC) remain challenging despite an increasingly comprehensive understanding of somatically altered oncogenic pathways. It is now clear that therapeutic agents with potential to impact the tumor immune microenvironment potentiate immune-orchestrated therapeutic benefit. Herein we evaluated the immunoregulatory properties of histone deacetylase (HDAC) and bromodomain inhibitors, two classes of drugs that modulate the epigenome, with a focus on key cell subsets that are engaged in an immune response. By evaluating human peripheral blood and NSCLC tumors, we show that the selective HDAC6 inhibitor ricolinostat promotes phenotypic changes that support enhanced T cell activation and improved function of antigen presenting cells. The bromodomain inhibitor JQ1 attenuated CD4+Foxp3+ T regulatory cell suppressive function and synergized with ricolinostat to facilitate immune-mediated tumor growth arrest, leading to prolonged survival of mice with lung adenocarcinomas. Collectively, our findings highlight the immunomodulatory effects of two epigenetic modifiers that, together, promote T cell-mediated anti-tumor immunity and demonstrate their therapeutic potential for treatment of NSCLC.
 
Overall design Single-cell comparison of vehicle (control) and HDAC inhibitor (ricolinostat)-treated tumor infiltrating T cells and macrophages
 
Contributor(s) Dries R, Adeegbe DO
Citation(s) 28408401
Submission date Apr 21, 2017
Last update date Mar 22, 2019
Contact name Ruben Dries
E-mail rubendries@gmail.com
Organization name DFCI
Department Computational Biology
Lab Longwood Center
Street address 360 Longwood Ave
City Boston
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (192)
GSM2585972 SSF1163_A1_ACY1215_1_KP2313_P3
GSM2585973 SSF1163_A1_Vehicle_1_KP2311_P3
GSM2585974 SSF1163_A10_ACY1215_1_KP2313_P3
This SubSeries is part of SuperSeries:
GSE98049 Synergistic Immunostimulatory Effects and Therapeutic Benefit of Combined Histone Deacetylase and Bromodomain Inhibition in Non-small Cell Lung Cancer.
Relations
BioProject PRJNA383813
SRA SRP104686

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE98048_processed_counts.txt.gz 1.4 Mb (ftp)(http) TXT
Processed data is available on Series record
Raw data are available in SRA

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