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| Status |
Public on Jun 20, 2017 |
| Title |
Real-Time PCR analysis of Embryoid Body differentiation of Human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
Human pluripotent stem cells were differentiated by size-controlled embryoid body (EB) formation in defined neutral, ectoderm, endoderm and mesoderm conditions. EB's were analysed at 4, 10 and 16 days post induction by real-time PCR using the Fludigm Deltagene primers and probes.
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| |
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| Overall design |
575 samples (with replicates) from undifferentiated pluripotent stem cells, Embryoid body samples at day 4, 10 and 16 post induction of differnetiation, in four conditions and Teratoma samples from the same cell lines
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| |
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| Contributor(s) |
Robson P, Halbritter F |
| Citation(s) |
29765017 |
| |
| Submission date |
Apr 19, 2017 |
| Last update date |
Jul 18, 2018 |
| Contact name |
Paul Gokhale |
| E-mail(s) |
p.gokhale@sheffield.ac.uk
|
| Phone |
0114 2222352
|
| Organization name |
University of Sheffield
|
| Department |
Biomedical Science
|
| Street address |
Western Bank
|
| City |
Sheffield |
| State/province |
N/A |
| ZIP/Postal code |
S10 2TN |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL23322 |
ISCI3 Custom human Delta Gene BioMark 96.96 Dynamic Array |
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| Samples (575)
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| This SubSeries is part of SuperSeries: |
| GSE97964 |
Assessing the developmental and malignant potential of human pluripotent stem cells |
|
| Relations |
| BioProject |
PRJNA383535 |
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