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Series GSE97839 Query DataSets for GSE97839
Status Public on Jul 05, 2017
Title DOC1-dependent recruitment of NURD reveals antagonism with SWI/SNF during epithelial-mesenchymal transition in oral cancer cells.
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The Nucleosome Remodeling and Deacetylase (NURD) complex is a key regulator of cell differentiation that has also been implicated in tumorigenesis. Loss of the NURD subunit DOC1 is associated with human oral squamous cell carcinomas (OSCC). Here, we show that restoration of DOC1 expression in OSCC cells leads to a reversal of epithelial-mesenchymal transition (EMT). This is caused by the DOC1-dependent recruitment of NURD to repress the Twist master regulators of EMT. NURD recruitment drives extensive epigenetic reprogramming, including eviction of the SWI/SNF remodeler, formation of inaccessible chromatin, H3K27 deacetylation, recruitment of PRC2 and KDM1A, followed by H3K27 methylation and H3K4 demethylation. Strikingly, depletion of SWI/SNF mimics the effects of DOC1 re-expression. Our results suggest that SWI/SNF and NURD function antagonistically to control chromatin state. We propose that disturbance of this dynamic equilibrium may lead to defects in gene expression that drive human cancer.
 
Overall design Examination of NURD binding with loss of NURD subunit DOC1.
 
Contributor(s) Mohd-Sarip A, Teeuwssen M, Bot AG, De Herdt MJ, Willems SM, Baatenburg de Jong RJ, van Riet J, van de Werken HJ, Oole E, van Ijcken WF, Looijenga LH, Zatreanu D, Bezstarosti K, Demmers JA, Fodde R, Verrijzer PC
Citation(s) 28683324
Submission date Apr 14, 2017
Last update date May 15, 2019
Contact name C. Peter Verrijzer
E-mail(s) c.verrijzer@erasmusmc.nl
Phone +31-104087461
Organization name Erasmus MC
Department Department of Biochemistry
Street address Dr Molewaterplein 50
City Rotterdam
ZIP/Postal code 3015 GE Rotterdam
Country Netherlands
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (8)
GSM2579159 lacZ-CHD4_ChIP-1
GSM2579160 lacZ-CHD4_ChIP-2
GSM2579161 lacZ-CHD4_ChIP-3
Relations
BioProject PRJNA382995
SRA SRP104112

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Supplementary file Size Download File type/resource
GSE97839_RAW.tar 810.0 Kb (http)(custom) TAR (of NARROWPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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