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Series GSE97672 Query DataSets for GSE97672
Status Public on May 05, 2017
Title Human monocyte-derived macrophage (MDM) cell transcriptome response to infection with H1N1, H3N2, and H5N1 influenza virus.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human monocyte-derived macrophages (MDM) serve as a model for resident alveolar macrophages (AM) in the human respiratory tract. mRNA-Seq analysis was used to profile the cellular transcriptome of MDM cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
 
Overall design Human monocyte-derived macrophages (MDM), obtained from healthy donor blood, were infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), or A/Vietnam/1203/04 (H5N1) HALo virus. Infected samples and time-matched mock-infected samples were collected in duplicates at 3, 6, 12, and 18 hrs post infection for mRNA-Seq analysis.
 
Contributor(s) Urbanowski M, Shaw M, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A
Citation(s) 30146161
NIH grant(s)
Grant ID Grant title Affiliation Name
U19 AI106754 Multiscale analysis of influenza host-pathogen interactions: Fluomics MOUNT SINAI SCHOOL OF MEDICINE Adolfo Garcia-Sastre
Submission date Apr 12, 2017
Last update date Mar 25, 2019
Contact name Max Chang
E-mail mchang@ucsd.edu
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (64)
GSM2575069 MDM-418_H3N2_MOI0_03h_1
GSM2575070 MDM-418_H3N2_MOI0_03h_2
GSM2575071 MDM-418_H3N2_MOI2_03h_1
Relations
BioProject PRJNA382632
SRA SRP103821

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97672_MDM_FPKM.csv.gz 9.8 Mb (ftp)(http) CSV
Raw data are available in SRA
Processed data is available on Series record

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