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| Status |
Public on May 05, 2017 |
| Title |
Human monocyte-derived macrophage (MDM) cell transcriptome response to infection with H1N1, H3N2, and H5N1 influenza virus. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human monocyte-derived macrophages (MDM) serve as a model for resident alveolar macrophages (AM) in the human respiratory tract. mRNA-Seq analysis was used to profile the cellular transcriptome of MDM cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
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| Overall design |
Human monocyte-derived macrophages (MDM), obtained from healthy donor blood, were infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), or A/Vietnam/1203/04 (H5N1) HALo virus. Infected samples and time-matched mock-infected samples were collected in duplicates at 3, 6, 12, and 18 hrs post infection for mRNA-Seq analysis.
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| Contributor(s) |
Urbanowski M, Shaw M, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A |
| Citation(s) |
30146161, 34556855 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U19 AI106754 |
Multiscale analysis of influenza host-pathogen interactions: Fluomics |
MOUNT SINAI SCHOOL OF MEDICINE |
Adolfo Garcia-Sastre |
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| Submission date |
Apr 12, 2017 |
| Last update date |
Dec 08, 2022 |
| Contact name |
Max Chang |
| E-mail(s) |
mchang@ucsd.edu
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| Organization name |
University of California, San Diego
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (64)
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| Relations |
| BioProject |
PRJNA382632 |
| SRA |
SRP103821 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE97672_MDM_FPKM.csv.gz |
9.8 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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