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Series GSE97672 Query DataSets for GSE97672
Status Public on May 05, 2017
Title Human monocyte-derived macrophage (MDM) cell transcriptome response to infection with H1N1, H3N2, and H5N1 influenza virus.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human monocyte-derived macrophages (MDM) serve as a model for resident alveolar macrophages (AM) in the human respiratory tract. mRNA-Seq analysis was used to profile the cellular transcriptome of MDM cells at multiple time points in response to infection with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), and A/Vietnam/1203/04 (H5N1) HALo virus. The Influenza A/Vietnam/1203/04 (H5N1) HALo mutant virus is an attenuated H5N1 virus generated from wild-type Influenza A/Vietnam/1203/04 (H5N1) virus as described in Steel, J., et al. J Virol. 2009 Feb; 83(4):1742-53.
Overall design Human monocyte-derived macrophages (MDM), obtained from healthy donor blood, were infected with influenza A/California/04/09 (H1N1), A/Wyoming/03/03 (H3N2), or A/Vietnam/1203/04 (H5N1) HALo virus. Infected samples and time-matched mock-infected samples were collected in duplicates at 3, 6, 12, and 18 hrs post infection for mRNA-Seq analysis.
Contributor(s) Urbanowski M, Shaw M, Heinz S, Benner C, Chang M, Pache L, Albrecht R, Garcia-Sastre A
Citation(s) 30146161, 34556855
NIH grant(s)
Grant ID Grant title Affiliation Name
U19 AI106754 Multiscale analysis of influenza host-pathogen interactions: Fluomics MOUNT SINAI SCHOOL OF MEDICINE Adolfo Garcia-Sastre
Submission date Apr 12, 2017
Last update date Dec 08, 2022
Contact name Max Chang
Organization name University of California, San Diego
Street address 9500 Gilman Drive
City La Jolla
State/province CA
ZIP/Postal code 92093
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (64)
GSM2575069 MDM-418_H3N2_MOI0_03h_1
GSM2575070 MDM-418_H3N2_MOI0_03h_2
GSM2575071 MDM-418_H3N2_MOI2_03h_1
BioProject PRJNA382632
SRA SRP103821

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97672_MDM_FPKM.csv.gz 9.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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