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Series GSE9767 Query DataSets for GSE9767
Status Public on Oct 16, 2008
Title Genotypic differences in water soluble carbohydrate metabolism in stem
Organism Triticum aestivum
Experiment type Expression profiling by array
Summary Water soluble carbohydrates (WSC, composed of mainly fructans, sucrose, glucose and fructose) deposited in wheat stems are important carbon sources for grain filling. Variation in stem WSC concentrations among wheat genotypes is one of the genetic factors influencing grain weight and yield under water-limited environments. Here, we describe the molecular dissection of carbohydrate metabolism in stems, at the WSC accumulation phase, of recombinant inbred SB (Seri/Babax) lines of Triticum aestivum differing in stem WSC concentrations. Affymetrix GeneChip analysis of carbohydrate metabolic enzymes revealed that the mRNA levels of two fructan synthetic enzyme families (sucrose:sucrose 1-fructosyltransferase and sucrose:fructan 6-fructosyltransferase) in the stem were positively correlated with stem WSC and fructan concentrations, while the mRNA levels of enzyme families involved in sucrose hydrolysis (sucrose synthase and soluble acid invertase) were inversely correlated with WSC concentrations. Differential regulation of the mRNA levels of these sucrose hydrolytic enzymes in SB lines resulted in genotypic differences in these enzyme activities. Down-regulation of sucrose synthase and soluble acid invertase in high WSC lines was accompanied by significant decreases in the mRNA levels of enzyme families related to sugar catabolic pathways (fructokinase and mitochondrion pyruvate dehydrogenase complex) and enzyme families involved in diverting UDP-glucose to cell wall synthesis (UDP-glucose 6-dehydrogenase, UDP-glucuronate decarboxylase and cellulose synthase), resulting in a reduction in cell wall polysaccharide contents (mainly hemicellulose) in the stem of high WSC lines. These data suggest that differential carbon partitioning in the wheat stem is one mechanism that contributes to genotypic variation in WSC accumulation.
We used Affymetrix GeneChip to dissect genotypic variation in carbohydrate metabolism related to water soluble carbohydrate accumulation in stems of wheat.
Keywords: Genotypic differences in water soluble carbohydrate metabolism in stem
Overall design 8 genotypes of recombinant inbred lines Seri M82 x Babax with 2 biological replicates per genotype. Grown in the field under rain-fed conditions
Contributor(s) Xue G, McIntyre CL, Shorter R, van Herwaarden AF
Citation(s) 18083795
Submission date Dec 04, 2007
Last update date Dec 04, 2018
Contact name Gang-Ping Xue
Phone 61 7 3214 2354
Fax 61 7 3214 2920
Organization name CSIRO
Department Plant Industry
Street address 306 Carmody Rd., St Lucia
City Brisbane
State/province Qld
ZIP/Postal code 4067
Country Australia
Platforms (1)
GPL3802 [wheat] Affymetrix Wheat Genome Array
Samples (16)
GSM246384 SB165_Stem_ rep1_at anthesis
GSM246385 SB165_Stem_ rep2_at anthesis
GSM246386 SB169_Stem_ rep1_at anthesis
BioProject PRJNA103701

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE9767_RAW.tar 71.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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