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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2007 |
| Title |
Embryonic stem cells with expanded CAG repeats (lorin-affy-mouse-501743) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Huntingtons disease (HD) is a devastating neurodegenerative disease, with out effective treatment. Despite significant advances, our understanding of how an expanded CAG repeat in the amino terminus of the large ubiquitously expressed HD gene product remains incomplete. Augmented adult neurogenesis in response to acute and chronic injury, including Huntingtons disease, has been demonstrated, but its role development, disease, and recovery is largely unknown, as are the factors controlling it. The HD gene product, huntingtin (htt) is know to interact with a number of transcription factors which subsequently influence gene expression. Understanding the factors involved in controlling neurogenesis in response to disease would bridge a significant knowledge gap and have tremendous therapeutic potential.
We propose to identify transcripts responsible for CAG repeat facilitated neurogenesis.
Our hypothesis is that expanded CAG repeats in the Huntingtons disease gene facilitates neurogenesis by altering transcription of genes critical in neurogenesis.
We have developed a model in which mouse embryonic stem cells (ESC) with expanded CAG repeats have facilitated neurogenesis. In this model more ESC with expanded CAG repeats transition to neuronal precursors and then develop into mature neurons more rapidly than control ESC. We propose to compare the gene expression profiles between ESC with expanded CAG repeats and control ESCs on days 4 and 6 of neuronal differentiation. Initial studies indicate that key transcriptional differences are occurring at these time points. Control ECS and a an ESC line with150 CAG repeats will be differentiated in triplicate, and RNA isolated form each replicate. It is anticipated that comparison of gene expression between the control and CAG repeat lines at each time point will identify transcripts involved in CAG repeat facilitated neurogenesis. Observed differences at day 4 may be more relevant to the transition from ESC to neuronal precursor whereas differences at day 6 may be more relevant to the neuronal precursor to neuron transition.
Keywords: time-course
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| Contributor(s) |
Lorincz MT |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 03, 2007 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Winnie Liang |
| E-mail(s) |
wliang@tgen.org
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| Organization name |
Translational Genomics
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| Street address |
445 N. Fifth Street
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| City |
Phoenix |
| State/province |
AZ |
| ZIP/Postal code |
85012 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM246309 |
nervous system: HM1 day 4 replicate 1_le1 |
| GSM246310 |
nervous system: HM1 day 4 replicate 2_le1 |
| GSM246311 |
nervous system: HM1 day 4 replicate 3_le1 |
| GSM246312 |
nervous system: CAG150 day 4 replicate 1_le1 |
| GSM246313 |
nervous system: CAG 150 day 4 replicate 2_le1 |
| GSM246314 |
nervous system: CAG 150 day 4 replicate 3_le1 |
| GSM246315 |
nervous system: CAG 150 day 6 replicate 1_le1 |
| GSM246316 |
nervous system: CAG 150 day 6 replicate 2_le1 |
| GSM246317 |
nervous system: CAG 150 day 6 replicate 3_le1 |
| GSM246318 |
nervous system: HM1 day 6 replicate 1_le1 |
| GSM246319 |
nervous system: HM1 day 6 replicate 2_le1 |
| GSM246320 |
nervous system: HM1 day 6 replicate 3_le1 |
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| Relations |
| BioProject |
PRJNA103687 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE9760_RAW.tar |
40.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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