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Series GSE97405 Query DataSets for GSE97405
Status Public on Apr 12, 2018
Title Parasitic helminths repress the canonical intestinal stem cell program
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The intestinal helminth parasite Heligmosomoides polygyrus initiates infection in mice by penetrating the duodenal mucosa, where it develops while surrounded by a multicellular granulomatous infiltrate before emerging into the intestinal lumen. We examined early H. polygyrus infection to assess the epithelial response to disruption of the mucosal barrier. Unexpectedly, intestinal stem cell markers, including Lgr5 and Olfm4, were completely lost in crypts overlying larvae-associated granulomas. We sought to identify the mechanism by which the H. polygyrus granuloma represses the activity of intestinal stem cells.
 
Overall design CD45-negative EpCAM-positive CD44-positive crypt cells were sorted from duodenum of mice infected with H. polygyrus for six days. Crypt cells from granuloma punch biopsies was compared to crypt cells from non-granuloma punch biopsies.
 
Contributor(s) Nusse YM, Savage AK, Klein OD, Locksley RM
Citation(s) 29950724
Submission date Apr 05, 2017
Last update date May 15, 2019
Contact name Adam K Savage
E-mail(s) adam.savage@ucsf.edu
Organization name University of California San Francisco
Lab S-1004
Street address 513 Parnassus Ave
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (9)
GSM2563989 Granuloma-associated crypt sort-316
GSM2563990 Granuloma-associated crypt sort-336
GSM2563991 Granuloma-associated crypt sort-359
Relations
BioProject PRJNA381696
SRA SRP103069

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Supplementary file Size Download File type/resource
GSE97405_Nusse_Savage-all_processed.txt.gz 3.5 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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