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Series GSE97311 Query DataSets for GSE97311
Status Public on Mar 13, 2019
Title Induced Mesenchymal stromal cells derived from iPS cells from aged individuals acquire a rejuvenation signature and a MSC-specific secretome
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Human induced pluripotent stem cells (iPSCs) - derived mesenchymal stromal cells (iMSCs) are a potentially useful cell type for circumventing ageing-related shortfalls associated with primary mesenchymal or skeletal stromal cells (MSCs/SSCs). To date, the extent of the reflection of ageing-hallmarks in iMSCs differentiated from iPSCs derived from elderly donors remains unclear. In this study, we show that fetal (55 days post conception) femur-derived MSCs and MSCs isolated from aged (60 – 74 years) donors differ in their transcriptome and secretome profile. Yet, iMSCs irrespective of donor age and cell type acquired a rejuvenation gene signature, specifically, INHBE, DNMT3B, POU5F1P1, CDKN1C, GCNT2; also present in pluripotent stem cells but not observed in the parental MSCs. Furthermore, dendrograms generated from the transcriptomes of iMSCs derived from the human embryonic stem cell line H1, fetal and aged MSCs always clustered together with the parental fetal femur-derived MSCs. In addition, these were distinct from MSCs isolated from aged donors when comparing gene ontologies (GOs) related to ageing processes. Critically, in terms of regenerative medicine applications, iMSCs re-acquired a similar secretome (e.g. SERPINE1, SDF-1a, HGF, IL6, IL10, THBS1) to that of the parental fetal MSCs, thus re-enforcing their capabilities of imparting context dependent paracrine signaling.
 
Overall design Total RNA obtained from induced mesenchymal stromal cells derived from iPS cells from aged and young individuals
 
Contributor(s) Wruck W, Megges M
Citation(s) 30885246
Submission date Apr 02, 2017
Last update date Apr 02, 2019
Contact name Wasco Wruck
E-mail(s) wasco.wruck@med.uni-duesseldorf.de
Organization name Universitätsklinikum Düsseldorf
Street address Moorenstr. 5
City Düsseldorf
ZIP/Postal code 40225
Country Germany
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (18)
GSM2561499 aged MSC (74y)
GSM2561500 iPSC (MSC, 74y, viral)
GSM2561501 iMSC (74y, viral)
Relations
BioProject PRJNA381360

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97311_Non-normalized_data.txt.gz 5.1 Mb (ftp)(http) TXT
GSE97311_RAW.tar 26.2 Mb (http)(custom) TAR
SRA Run SelectorHelp
Raw data is available on Series record
Processed data included within Sample table

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