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Series GSE97232 Query DataSets for GSE97232
Status Public on Jun 27, 2018
Title p21 misexpression in the left growth plates
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To study mechanisms of long bone growth regulation, p21 misexpression was induced in the left hindlimb of mouse embryos using an intersectional approach that requires both Cre and (r)tTA activity. Doxycycline was provided to the pregnant female at embryonic day (E)12.5 to activate the transgene, and embryos were collected at E17.5. Distal femur and proximal tibia growth plates were dissected out, keeping left and right separated, deprived of perichondrium and flash frozen. After RNA extraction, mRNA libraries were prepared and all samples were deep sequenced in parallel
 
Overall design 6 samples (left and right growth plates from embryos #386, #387, #388) were sequenced in parallel
 
Contributor(s) Joyner AL, Roselló-Díez A
Citation(s) 29944650
NIH grant(s)
Grant ID Grant title Affiliation Name
R21 HD083860 A novel genetic approach to perturb and study coordination of organ growth Sloan Kettering Institute for Cancer Research ALEXANDRA L. JOYNER
Submission date Mar 30, 2017
Last update date May 15, 2019
Contact name Alexandra L Joyner
E-mail(s) joynera@mskcc.org
Organization name Sloan Kettering Institute
Street address 1275 York Avenue. Box 511
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM2559977 386L
GSM2559978 386R
GSM2559979 387L
Relations
BioProject PRJNA381042
SRA SRP102791

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97232_RAW.tar 9.2 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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