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Series GSE97177 Query DataSets for GSE97177
Status Public on Mar 30, 2017
Title Genome-wide multi-omics profiling reveals extensive genetic complexity in 8p11-p12 amplified breast carcinomas [expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Transcriptomic profiling of human breast tumors.

Genomic and expression profiling using 38K BAC array-CGH and Illumina HT-12 beadchips were performed on 53 invasive breast tumors to assess the impact of gene dosage on gene expression patterns and the effect of other mechanisms on transcriptional levels. Array-CGH results was validated by FISH using tumors showing 8p11-p12 DNA amplification and expression profiling was confirmed using qPCR for 11 transcripts. Low-level gain, high-level gain/amplification, heterozygous loss and homozygous deletion (henceforth referred to as gain, amplification, loss and deletion) were defined as log2 ratio thresholds set at +0.2, >= +0.5, -0.2 and <=-1.0, respectively.
Overall design Total RNA was isolated from fresh-frozen tissue samples
Contributor(s) Parris TZ, Rönnerman EW, Engqvist H, Biermann J, Truvé K, Nemes S, Forssell-Aronsson E, Kovács A, Karlsson P, Helou K
Citation(s) 20551037, 24662924, 28877888, 29844878
Submission date Mar 29, 2017
Last update date Feb 10, 2020
Contact name Toshima Z. Parris
Organization name University of Gothenburg
Department Department of Oncology
Lab Sahlgrenska Cancer Center
Street address Medicinaregatan 1G, 6th floor
City Gothenburg
ZIP/Postal code 41390
Country Sweden
Platforms (1)
GPL6947 Illumina HumanHT-12 V3.0 expression beadchip
Samples (53)
GSM2556314 Tumor_4296 (expr)
GSM2556316 Tumor_4302 (expr)
GSM2556318 Tumor_5582 (expr)
This SubSeries is part of SuperSeries:
GSE97293 Genome-wide multi-omics profiling reveals extensive genetic complexity in 8p11-p12 amplified breast carcinomas
BioProject PRJNA380897

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE97177_Non-normalized_data.txt.gz 7.8 Mb (ftp)(http) TXT
GSE97177_RAW.tar 119.0 Mb (http)(custom) TAR (of IDAT)
Raw data provided as supplementary file
Processed data included within Sample table
Raw data are available on Series record

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