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| Status |
Public on Apr 27, 2017 |
| Title |
EBF2-regulated epigenome in brown adipose tissue [ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
EBF2 activates the expression of brown fat-selective genes in adipocytes, but whether EBF2 regulates these genes via direct binding was unknown. To address this question, we analyzed the genome-wide binding profile of EBF2 in BAT using chromatin immunoprecipitation followed by deep sequencing (ChIP-seq). To determine if EBF2 is required for the activity of lineage-specific enhancers, we examined the levels of PPARĪ³, RNA Polymerase II (Pol II), and H3K27ac at brown fat-specific genes in wildtype (WT) and Ebf2 KO BAT.
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| Overall design |
Brown adipose tissues were collected from Ebf2 knockout and wildtype mice and ChIP-seq was performed for EBF2, PolII, PPARy, and H3K27ac.
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| Contributor(s) |
Shapira SN, Lim H, Rajakumari S, Sakers AP, Ishibashi J, Harms MJ, Won K, Seale P |
| Citation(s) |
28428261 |
| |
| Submission date |
Mar 28, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hee-Woong Lim |
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Department |
Division of Biomedical Informatics
|
| Street address |
3333 Burnet Ave. MLC 7024
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE97116 |
Ebf2 Knockout Brown Adipose Tissue |
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| Relations |
| BioProject |
PRJNA380852 |
| SRA |
SRP102658 |
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