|
Status |
Public on Jun 26, 2017 |
Title |
Effects of arsenic and Pseudomonas aeruginosa infection on gene expression in human airway epithelial cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Purpose: To determine effects of arsenic on gene expression in polarized primary human bronchial epithelial (HBE) cells and impact on transcriptional response to Pseudomonas aeruginosa infection Methods: mRNA profiles of HBE cells from 6 donors exposed to 0, 5, 10 or 50 ug/L total arsenic +/- Pseudomonas aeruginosa (48 samples) were generated using Illumina sequencing, aligned in CLC Genomics workbench and analyzed for DE in EdgeR Findings: 20-30 million reads were mapped per sample and transcripts were identifed that were significantly differentially expressed in response to arsenic and Pseudomonas aeruginosa
|
|
|
Overall design |
Gene expression profiles of HBE cells from 6 donors exposed to three concentrations of arsenic +/- Pseudomonas were generated using mRNA sequencing
|
|
|
Contributor(s) |
Stanton BA |
Citation(s) |
28625800 |
Submission date |
Mar 26, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Britton C Goodale |
E-mail(s) |
Britton.C.Goodale@Dartmouth.edu
|
Organization name |
Geisel School of Medicine at Dartmouth
|
Department |
Microbiology and Immunology
|
Lab |
Stanton
|
Street address |
604 Remsen Building
|
City |
Hanover |
State/province |
NH |
ZIP/Postal code |
03755 |
Country |
USA |
|
|
Platforms (1) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
|
Samples (48)
|
|
Relations |
BioProject |
PRJNA380496 |
SRA |
SRP102483 |