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Series GSE96850 Query DataSets for GSE96850
Status Public on Jun 20, 2017
Title Generational Comparisons (F1 versus F3) of Vinclozolin Induced Epigenetic Transgenerational Inheritance of Sperm Differential DNA Methylation Regions (Epimutations) Using MeDIP-Seq
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary The agricultural fungicide vinclozolin exposure of a gestating female rat has previously been shown to promote transgenerational disease and epimutations in F3 generation (great-grand-offspring) animals. The current study was designed to investigate the actions of direct fetal exposure on the F1 generation rat sperm DMRs compared to the F3 transgenerational sperm DMRs. The F1 generation DMRs were found to be fewer in number and for the most part distinct from the F3 generation epimutations. Therefore, the direct exposure induced F1 generation DMRs appear to promote alterations in germ cell development that lead to the programming of the F3 generation epimutations, but are distinct between the generations.
Overall design F0 generation gestating female rats were treated with either vinclozolin or dimethyl sulfoxide (vehicle). The rats were then raised and bred to the F3 generation. Genome wide methylation levels were assessed for both the F1 and F3 generation rats. This was done by pooling three rat individual samples into a single sample pool. Three pools for each treatment in each generation were collected. The methylation for each pool was measured using MeDIP-seq on an Illumina HiSeq 2500.

DOI: 10.1093/eep/dvx016
Contributor(s) Beck D, Sadler-Riggleman I, Skinner MK
Citation Beck D, Ingrid S, Michael SK. Generational comparisons (F1 versus F3) of vinclozolin induced epigenetic transgenerational inheritance of sperm differential DNA methylation regions (epimutations) using MeDIP-Seq. Environmental Epigenetics. 2017 July 20; 3(3):dvx016.
Submission date Mar 21, 2017
Last update date May 15, 2019
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (12)
GSM2545057 F1C1
GSM2545058 F1C2
GSM2545059 F1C3
BioProject PRJNA379935
SRA SRP102230

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Supplementary file Size Download File type/resource
GSE96850_F1.results.csv.gz 595.6 Mb (ftp)(http) CSV
GSE96850_F3.results.csv.gz 514.8 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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